I found that almost every paper that shows in situ hybridization, uses DIG-UTPs for labeling with fluorescence anti-DIG antibodies.
Is there any advantage over Biotin-UTPs and fluorescence avidin labeling?
I work with zebrafish embryos that express APEX2, and when I soak them with Biotin-tyramide in DMSO (10% for 2 hours, etc.) they die. is there a less toxic way to quench the embryos with biotin?
20 March 2023 9,263 0 View
How do I know if my in situ hybridization probes binds mRNA and not genomic locus?
18 March 2023 2,057 7 View
Hi I'm looking for the subcellular location of some maternal transcripts in zebrafish embryos, before the genome activation (MZT). I use 'Molecular Instruments' HCR-FISH kit (probes and...
17 March 2023 2,418 0 View
I have a plasmid that I want to delete a segment from it. I designed primers for linearization of the vector without the unwanted sequence, and with overlapped ends. I heard that Gibsson assembly...
17 January 2023 7,942 4 View
Octakis(trimethylsiloxy)silsesquioxane is reported to be sparingly soluble (0.61 g/L 25°C Calc). I tried using organic solvent such as toluene, hexane, DCM and chloroform, however the powder...
13 January 2021 1,323 3 View
Hello all, I have TEMPO radical and I want to manipulate it in a way the it will no longer be a stable radical, do you know if that can be done? Alternatively, do you know of a material to TEMPO...
14 June 2020 4,455 0 View
I have stuck frit inside an HPLC column I want to remove, anyone knows how to remove it?
16 February 2019 5,876 6 View
Hello all, I'm using an home-built HPLC column and the pressure goes to the maximum even with very low flow (0.01 ml/min). Do you have any suggestions as to how to reduce pressure? Perhaps I...
03 September 2018 7,763 2 View
the silica nanoprticles may be dissolved to sodium silicate
07 July 2018 1,468 3 View
Is increasing large scale fading coefficients across the cells causes pilot contamination in massive MIMO? When orthogonal pilots are used across cell?
22 January 2018 7,523 4 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
How to calculate the molar proportions of the oxides from the XRF analyses?
02 August 2024 2,565 2 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I’m doing cell-surface biotinylation with HEK293 cells and after all the washing and biotin incubation steps (washing with PBS ph8, neutralizing unreacted biotin with 40mM Tris ph8) many of my...
23 July 2024 4,740 3 View
I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?
23 July 2024 8,059 1 View