I need to attain high volumes of trichderma spores to be used as inculum for pilot scale fermentation..
The ususal method scaling up with broth , like PDB, is not desirable for industries.
I need 0.2% inoculum size in terms of spore of at least 10E6 count. Hence looking for a media.
My colleague uses wheat bran: Article Remediation of Phytophthora ramorum -infested soil with Tric...
Hi, Potato dextrose agare is a good media for producing spore for Trichoderma.
There is a good review of Trichoderma at Article Trichoderma Research in the Genome Era
. As several have reported, spore production is generally enhanced with low nutrient conditions. In addition, factors such as low pH in the medium (e.g.Article Statistical culture-based strategies to enhance chlamydospor...
) can be important. Growth conditions also are important, at least for conidia. Most Trichoderma spp. show photoinduction of conidia (e.g.Article Trichoderma in the light of day - Physiology and development
Check this reference:
Article Omics analyses of Trichoderma reesei CBS999.97 and QM6a indi...
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=9&ved=2ahUKEwjVqJe19avjAhVEU1AKHZR1COIQFjAIegQICBAC&url=https%3A%2F%2Fpdfs.semanticscholar.org%2F2fdd%2Fba86dc6f5c5e6fb9dee7919980a1799ecd9e.pdf&usg=AOvVaw2cgwA0kS1TihDkXPSLKGuI
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=16&cad=rja&uact=8&ved=2ahUKEwiN0KzhlK3jAhUEx4UKHYOoCBU4ChAWMAV6BAgFEAE&url=https%3A%2F%2Fwww.frontiersin.org%2Farticles%2F303441&usg=AOvVaw0tcucGBmJ9FIAQu3zzrk15
You can use PDA (Potato Dextrose Agar), PCA (Potato Carrot Agar), CMA (Corn Meal Agar) or MEA (Malt Extract Agar). All these media are good for Trichoderma sporulation.
Potato Dextrose Agar (PDA) and Malt Extract Agar (MEA) are good medium for isolation
You can use PDA , PCA and Panicum miliaceum seeds(Millet).
Best Regard.
May I recommend MAlt Extract Agar MEA or Broth MEB Media
Very goo for Sporulating reesei / Potato Dextrose is a good media too
------ Trichoderma development/ sporulation depends tightly on Light and nutrition ...
Best
according to my research the PDA and Malt media are the best for mushroom growth
Malt Extract Agar (MEA) is a good medium for sporulating Trichoderma.
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