Is FFPE DNA unstable for conventional PCR?

I am trying to amplify target sequence using FFPE DNA. Initially, I managed to get the target band I want although it is weak. However, when I try to repeat the test using same formula, no band...

31 December 2014 7,011 11 View

Does anyone have good primers for APC gene at MCR?

I am using FFPE samples to detect APC mutation at MCR. Are there any good papers I can refer my work to?

08 September 2014 6,349 0 View

What is the difference between HCT116 (FBXW7 +/+), HCT116 (FBXW7 +/-) and HCT116 (FBXW7 -/-)? Is HCT116 (FBXW7 +/+) wildtype HCT116?

How can FBXW7 mutation can be obtained from a HCT116 (FBXW7 -/-) if the FBXW7 gene has been deleted?

04 May 2014 9,638 3 View

Does anyone have experience scoring IHC for tissue staining using MLH1, MSH2 or MSH6 antibodies?

I have done the staining of above antibodies on human colon tissue but have difficulties scoring them. How to determine whether the tissue sample is positively or negatively stain. According to...

03 April 2014 2,676 1 View

Can anyone help with PCR primer overlap with mutation site?

My samples are FFPE tissue, so the amplicon must be

01 February 2014 1,183 5 View

How to detect chromosomal Instability(CIN)? What are the methods available?

I am doing cancer study and I might need to do CIN. Is there any reference, journals, books available that can give me some ideas?

31 December 2013 6,240 6 View

Can I still use the tissue for IHC if I immersed it in 70% ethanol instead of xylene?

I confused the rehydration step with the dehydration step by immersing tissue in 70% ethanol instead of xylene. Can my tissue still be used?

11 December 2013 1,110 6 View

How should I do scoring for cytoplasmic staining in immunohistochemistry?

The standard method is to count the positive staining. What if my staining needs to show loss of expression? Also, usually, when there is a loss of expression, there is a whole area of loss. I do...

10 November 2013 3,625 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View