Boon Lee Chai

9 Questions 15 Answers 0 Followers

Questions related from Boon Lee Chai

Is FFPE DNA unstable for conventional PCR?

I am trying to amplify target sequence using FFPE DNA. Initially, I managed to get the target band I want although it is weak. However, when I try to repeat the test using same formula, no band...

01 January 2015 7,096 11 View

Does anyone have good primers for APC gene at MCR?

I am using FFPE samples to detect APC mutation at MCR. Are there any good papers I can refer my work to?

09 September 2014 6,375 0 View

Can anyone suggest a good anti-APC C-terminal antibody?

Besides HT29 as the negative control for anti-APC C-terminal antibody, is there any other colorectal cell lines that can be used? Could we possibly use SW480?

06 June 2014 7,305 2 View

What is the difference between HCT116 (FBXW7 +/+), HCT116 (FBXW7 +/-) and HCT116 (FBXW7 -/-)? Is HCT116 (FBXW7 +/+) wildtype HCT116?

How can FBXW7 mutation can be obtained from a HCT116 (FBXW7 -/-) if the FBXW7 gene has been deleted?

05 May 2014 9,666 3 View

Does anyone have experience scoring IHC for tissue staining using MLH1, MSH2 or MSH6 antibodies?

I have done the staining of above antibodies on human colon tissue but have difficulties scoring them. How to determine whether the tissue sample is positively or negatively stain. According to...

04 April 2014 2,703 1 View

Can anyone help with PCR primer overlap with mutation site?

My samples are FFPE tissue, so the amplicon must be

02 February 2014 1,213 5 View

How to detect chromosomal Instability(CIN)? What are the methods available?

I am doing cancer study and I might need to do CIN. Is there any reference, journals, books available that can give me some ideas?

01 January 2014 6,280 6 View

Can I still use the tissue for IHC if I immersed it in 70% ethanol instead of xylene?

I confused the rehydration step with the dehydration step by immersing tissue in 70% ethanol instead of xylene. Can my tissue still be used?

12 December 2013 1,140 6 View

How should I do scoring for cytoplasmic staining in immunohistochemistry?

The standard method is to count the positive staining. What if my staining needs to show loss of expression? Also, usually, when there is a loss of expression, there is a whole area of loss. I do...

11 November 2013 3,653 4 View