I am using FFPE samples to detect APC mutation at MCR. Are there any good papers I can refer my work to?
I am trying to amplify target sequence using FFPE DNA. Initially, I managed to get the target band I want although it is weak. However, when I try to repeat the test using same formula, no band...
31 December 2014 7,148 11 View
Besides HT29 as the negative control for anti-APC C-terminal antibody, is there any other colorectal cell lines that can be used? Could we possibly use SW480?
05 June 2014 7,332 2 View
How can FBXW7 mutation can be obtained from a HCT116 (FBXW7 -/-) if the FBXW7 gene has been deleted?
04 May 2014 9,695 3 View
I have done the staining of above antibodies on human colon tissue but have difficulties scoring them. How to determine whether the tissue sample is positively or negatively stain. According to...
03 April 2014 2,727 1 View
My samples are FFPE tissue, so the amplicon must be
01 February 2014 1,250 5 View
I am doing cancer study and I might need to do CIN. Is there any reference, journals, books available that can give me some ideas?
31 December 2013 6,320 6 View
I confused the rehydration step with the dehydration step by immersing tissue in 70% ethanol instead of xylene. Can my tissue still be used?
11 December 2013 1,165 6 View
The standard method is to count the positive staining. What if my staining needs to show loss of expression? Also, usually, when there is a loss of expression, there is a whole area of loss. I do...
10 November 2013 3,686 4 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...
25 July 2024 6,517 3 View