I am using FFPE samples to detect APC mutation at MCR. Are there any good papers I can refer my work to?
I am trying to amplify target sequence using FFPE DNA. Initially, I managed to get the target band I want although it is weak. However, when I try to repeat the test using same formula, no band...
31 December 2014 7,011 11 View
Besides HT29 as the negative control for anti-APC C-terminal antibody, is there any other colorectal cell lines that can be used? Could we possibly use SW480?
05 June 2014 7,277 2 View
How can FBXW7 mutation can be obtained from a HCT116 (FBXW7 -/-) if the FBXW7 gene has been deleted?
04 May 2014 9,638 3 View
I have done the staining of above antibodies on human colon tissue but have difficulties scoring them. How to determine whether the tissue sample is positively or negatively stain. According to...
03 April 2014 2,676 1 View
My samples are FFPE tissue, so the amplicon must be
01 February 2014 1,183 5 View
I am doing cancer study and I might need to do CIN. Is there any reference, journals, books available that can give me some ideas?
31 December 2013 6,240 6 View
I confused the rehydration step with the dehydration step by immersing tissue in 70% ethanol instead of xylene. Can my tissue still be used?
11 December 2013 1,110 6 View
The standard method is to count the positive staining. What if my staining needs to show loss of expression? Also, usually, when there is a loss of expression, there is a whole area of loss. I do...
10 November 2013 3,625 4 View
I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...
03 March 2021 8,066 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...
01 March 2021 360 7 View
I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...
28 February 2021 606 3 View
hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?
28 February 2021 1,254 3 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View