Hi Francesca

Look at this paper, it can help you .

J Pharmacol Exp Ther. 1988 Jan;244(1):100-5.

Effects of prolonged phenylephrine infusion on cardiac adrenoceptors and calcium channels.

Gengo PJ1, Bowling N, Wyss VL, Hayes JS.

Chronic (6-day) infusion (3 mg/kg/hr) utilizing Alzet mini-osmotic pumps maintained plasma concentrations of phenylephrine at 1.0 microgram/ml,

Have a good day

jacques

Another one,

Hypertension. 1999; 33: 396-401 ,

β-Adrenergic Receptor Kinase-1 Levels in Catecholamine-Induced Myocardial Hypertrophy

Regulation by β- but not α1-Adrenergic Stimulation

Guido Iaccarino,Paul C. Dolber,Robert J. Lefkowitz,Walter J. Koch

Pumps were filled with ISO, PE, or vehicle (0.02% ascorbic acid) and were set to deliver ISO at 30 mg · kg−1 · d−1 and PE at 100 mg · kg−1 · d−1 for 14 days each.

and finally

Journal of the American College of Cardiology Vol. 38, No. 2, 2001

Regulation of Myocardial bARK1

Expression in Catecholamine-Induced

Cardiac Hypertrophy in Transgenic

Mice Overexpressing a1B-Adrenergic Receptors

Guido Iaccarino, MD,*† Janelle R. Keys, PHD,† Antonio Rapacciuolo, MD,* Kyle F. Shotwell, BS,†

Robert J. Lefkowitz, MD,*‡§ Howard A. Rockman, MD,* Walter J. Koch, PHD†

Thanks Jacques but the first reference is on rats and the last 2 do not show the pressure response over time. I need to find a dose for mice that elicits a gradual pressure response similar to angII 1mg/kg/d. Thanks anyway.

 Hello Francesca,

I was wondering if you end up finding the dose for Phenylephrine to infuse in mice.

I would appreciate if you can share the protocol.

Thanks :-)

HI Hiba,

we tried phenyl at 3, 12 and 60 mg/kg/d but blood pressure increased very modestly compared to baseline. The problem was a massive decrease in HR (from 650 to 450 bpm, all measured by radiotelemetry). We then decided to use norepinephrine 10mg/kg/d, which did not have effect on HR and increased BP to about 140mmHg. Hope this helps!

Hi Francesca,

Thank you for your fast reply. Was the decrease in heart rate at the lower dose too?

Did you have a chance to check the heart tissue and see if there was a direct effect on cardiomyocytes (hybertrophy) or fibrosis?  

Thanks again for your help and best of luck!

We did not see the HR effect with 3mg/kg/d but BP did not change either. Both 12 and 60 mg/kg/d doses gave the bradycardic effect and very modest and transient increases in BP. We did not look at heart tissue. Good luck.

1mM

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