Can anyone recommend an anti-human RORgt- antibody for flow cytometry?

Hi, I'm looking for a suitable ab that stains human RORgt and that works in flow cytometry. Thanks!

01 February 2019 7,572 3 View

What is the best method for transfection of primary human naive CD4 T cells?

Hi, I am planning shRNA transfection experiments using primary human naive CD4 T cells. A specific requirement for my experiment would be that the cells should not be TCR activated (or at least...

03 April 2018 227 4 View

What is the surface expression level of CD66b on circulating human neutrophils?

Hi, I am wondering about the surface levels of CD66b on unstimulated human neutrophils, analyzed ex vivo in whole blood using flow cytometry. The literature appears controversial, ranging from a...

02 March 2018 6,461 5 View

Are there human cells that express detectable amounts of surface IL-12R protein at baseline?

I am looking for human cells (primary or cell line) that constitutively express both chains of the heterodimeric IL-12 receptor (IL-12Rb1 and IL-12Rb2) at their cell surface. Detection of the...

07 August 2017 8,876 2 View

What is the expression level of IFNAR on human T cells?

Does anyone have experience with IFNAR staining on primary human T cells for flow cytometry? Staining in my hands is really poor and although I don't expect high levels of expression I am not...

01 February 2017 2,012 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View