Does anyone have experience with IFNAR staining on primary human T cells for flow cytometry? Staining in my hands is really poor and although I don't expect high levels of expression I am not quite sure what the staining should look like...thanks!
All cell types express IFNAR and respond to type I IFN treatment. However, the extent of expression varies with cell-type. In our hands, we can detect the IFNAR on human and murine T cells by immunoblotting. The ability to detect IFNAR by flow-cytometery on T cells varies to great extent. Further, we do not trust commercially available antibodies that are available for detection by flow (due to cross-reactivity) to quantitate IFNAR. Therefore, it may be difficult to find a paper where the detection is well documented.
Thanks to a very rare and interesting feature comes-question but also very rare well-documented response, this is very useful to me!
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology