Hi,

I am planning shRNA transfection experiments using primary human naive CD4 T cells. A specific requirement for my experiment would be that the cells should not be TCR activated (or at least not too much for too long) since I want to keep them in the naive state as far as possible. I am thinking of culturing the cells in the presence of low dose IL-7 and/or IL-2 prior to transfection to induce proliferation without triggering conversion to memory cells. Is that a good idea? From what I've read and heard, electroporation seems to be the method of choice...I'd be very intersted in anyone's experience or advice in this aera.

Thanks!