I am looking for human cells (primary or cell line) that constitutively express both chains of the heterodimeric IL-12 receptor (IL-12Rb1 and IL-12Rb2) at their cell surface. Detection of the IL-12R should be achievable by flow cytometry.
Thanks!
Amended on 16 August 2017
I have searched for IL-12R in vain among my liver and serum specimens by using new quantitative proteomics of PDMD method (please see file; HepG2 fucoidan).
LC tissue (with leprosy) has Interleukin-32/Natural killer cells protein 4 at 1.5, and Interleukin-4/B-cell stimulatory factor 1 at 0.97 μg/mg of tissue protein, respectively.
LC tissue (No.6) has Interleukin-1 alpha/IL-1 alpha at 2.1, and HCC tissue (No.6) has Interleukin-7 receptor alpha chain/IL-7RA at 0.29 μg/mg of tissue protein, respectively.
Serum of biotin deficiency (with GSD-1b) has Interleukin-9 receptor at 4.5 μg/mg of serum protein.
Serum of healthy female (52y) has Interleukin-31 receptor subunit alpha at 3.5 μg/mg of serum protein.
It should be known that the high-price flow cytometry is not a quantitative detector at all, and a uselessness machine. Flow cytometer does not obey the Lambert-Beer's Law at all. Then, determination of expression of IL-12R should be performed by the newly developed proteomics of PDMD method, which is based upon the chemical Edman degradation-HPLC method (automated microsequening machine may be costly, but lower price than the flow cytometry ). This is not my propaganda, but you should utilize the quantitative machine which obeys the Lambert-Beer's Law such as HPLC photometric method not the notorious HPLC-MS method. Then, you would get the reliable and fruitful results.
Dear Dr Hayakawa,
Thank you for your prompt response, I appreciate your comment about the usefulness of flow cytometry as quantitative detector.
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