Christian Hundhausen

6 Questions 23 Answers 0 Followers

Questions related from Christian Hundhausen

Can anyone recommend an anti-human RORgt- antibody for flow cytometry?

Hi, I'm looking for a suitable ab that stains human RORgt and that works in flow cytometry. Thanks!

02 February 2019 7,632 3 View

Can anyone recommend an ELISA for measuring tocilizumab concentrations in human serum?

Hi, I am looking for some advice on measuring tocilizumab levels in human serum. Can anyone recommend a commercially availbale ELISA kit? Thanks

09 September 2018 8,430 2 View

What is the best method for transfection of primary human naive CD4 T cells?

Hi, I am planning shRNA transfection experiments using primary human naive CD4 T cells. A specific requirement for my experiment would be that the cells should not be TCR activated (or at least...

04 April 2018 283 4 View

What is the surface expression level of CD66b on circulating human neutrophils?

Hi, I am wondering about the surface levels of CD66b on unstimulated human neutrophils, analyzed ex vivo in whole blood using flow cytometry. The literature appears controversial, ranging from a...

03 March 2018 6,520 5 View

Are there human cells that express detectable amounts of surface IL-12R protein at baseline?

I am looking for human cells (primary or cell line) that constitutively express both chains of the heterodimeric IL-12 receptor (IL-12Rb1 and IL-12Rb2) at their cell surface. Detection of the...

08 August 2017 8,934 2 View

What is the expression level of IFNAR on human T cells?

Does anyone have experience with IFNAR staining on primary human T cells for flow cytometry? Staining in my hands is really poor and although I don't expect high levels of expression I am not...

02 February 2017 2,079 2 View