Hello everyone. I am working on my LV concentration porject, I use 500Kda hollowfiber to do it. The HCP and BSA removal rate is nearly 80%, but last time, I failed to run it. The HCP hasn't been removed as usual, the removal rate is only 1%. Here is my operation parameters.

membrane:PES

Area:0.5cm²

inlet pressure:0.02bar

outlet and retentate pressure is 0

This is the 2nd use, the first run of this column is good as usual. There is no block during the operation time.

It is strange, does anyone can help me?

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