Is annealing should be done again after a year of nano-particles fabrication and they are found to be agglomerated?

I have prepared CeO2 based nano-particles. due to some technical issues some of the properties like electronic structure and magnetic properties are pending. the samples were prepared...

08 October 2020 769 3 View

How to transform a dummy variable for Log-Log regression?

My Marketing Mix Modeling data contains some categorical variables which I convert into dummy variables. Before trying to create a Log-Log model I plan on replacing the 0 values with 1 and 1...

12 April 2020 8,251 7 View

Is there any study to prove "toilet plume" is a health hazards?

Toilet plume/ aerosolised faeces contain Bacteria and pathogens, but are there any research papers or experiments to prove that they are a health hazard.

30 January 2020 1,270 3 View

Kindly give suggestions for PhD topic on Electric vehicles?

I am looking for research topic related to Electric Vehicle , something related to optimisation. Like application of metaheuristics to it. I am much confused which one to choose for research like...

19 October 2019 4,744 5 View

How to calculate solar power output using beam irradiance and diffuse irradiance for given rated PV plant ?

i am looking for formula to calculate solar power output on hourly basis for PV plant with given rating using irradiance and diffuse irradiance. I have hourly data for irradiance and diffuse...

05 October 2019 1,385 7 View

How to model linear snap through buckling in Abaqus ?

Hello, I am currently trying to model a 2D wire Abaqus model as a beam to study linear snap buckling. I am using eigenvalue buckling concept and was able to get the 1st eigenmode value and...

11 June 2018 896 2 View

Building Extraction from High Resolution Satellite Imagery ?

Hello All, I have trying to understand the potential of building rooftop extraction, i.e to understand what societal benefit it has to it attached, as Just extraction of building won't help much...

13 February 2018 3,956 3 View

What is the best buffer recipe for genome extraction from tissue?

I work with zebrafish adults and larvae and am trying to characterise mutations from CRISPR and ENU mutants. I either extract the genome from entire larvae between 3 and 5dpf or adult fin clips....

01 April 2017 428 1 View

Can the same ELISA assay kit for a hormone work across all species?

I am testing zebrafish and Xenopus laevis for reduced hormone levels. It is the same hormone in both species. My method of choice is ELISA. However, when I researched for the available kits, they...

29 August 2016 3,550 2 View

Can we do total RNA extraction from tissues preserved in 4% PFA?

I have been doing a lot of total RNA extraction from wild type zebrafish. However, with other experiments going on simultaneously, I often find very little time left for dissecting the fish and...

05 July 2016 8,019 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View