Carolin Ulbricht

3 Questions 4 Answers 0 Followers

Questions related from Carolin Ulbricht

LPS- B cell activation only works once in a while??

I was wondering if anybody could give some tips for B cell culture (primary splenic B cells, MACS purified, 5x10^5/ml + 8ug/ml LPS, + 20ng/ml IL-4, in RPMI, 1-2 days). It seems that I can use...

14 July 2020 6,559 0 View

Buffers needed for Annexin V staining?

Hello you all, we want to perform an Annexin V and PI staining on our B cell culture, does anybody know if using the commercial buffer (biolegend in our case) is absolutely necessary? Or can we...

15 May 2019 2,679 3 View

Can anyone provide a protocol or source for how to easily obtain plasma membrane sheets?

I want to couple NP to PMSs so that it can be recognized by NP-spec. B cells.

21 August 2018 2,052 2 View