In Abaqus, is it possible to remove elements which are too complex for meshing from a sequence obtained by getByBoundingBox() ?

Hello everyone, I'm trying to mesh a structure with complex partitions using mesh controls. When doing it on the user interface I'm first selecting all cells. In the following, I have to accept...

03 February 2021 8,198 1 View

How to compare expected and counted values statistically with different p values for each genotype?

Hey there, I am currently working on the statistics of counted and expected genotypes in mice litter of a mutant mouse model. I have two different mutated genes, both alone and in combination, one...

03 February 2021 6,408 2 View

Can a mixed background in mice reduce cre recombinase activity in comparison to a pure Bl6 background?

Our transgenic mice show a really strong phenotype which differs a lot from results of another group working with the same transgene. As our mice are on a pure C57Bl6J background and theirs was on...

24 September 2020 1,792 3 View

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Dear all, I have the following problem: I want to run a co-citation analysis of a certain set of publications. However, some of them are not part of the Web of Science database. Is there a...

13 December 2019 9,581 7 View

Buffers needed for Annexin V staining?

Hello you all, we want to perform an Annexin V and PI staining on our B cell culture, does anybody know if using the commercial buffer (biolegend in our case) is absolutely necessary? Or can we...

14 May 2019 2,538 3 View

How much digested plasmid DNA can be loaded onto an agarose gel for subsequent gel extraction?

I'm digesting a 11 kbp plasmid, resulting in three fragments (should be around 5, 4 and 2 kbp, respectively). What amount of digested DNA (in ng) would you recommend to be loaded onto a 1% agarose...

28 April 2019 350 3 View

No bands in Western Blot that worked before

22 May 2017 8,698 10 View

Can anyone help me precipitation of temperate phages for DNA extraction?

I want to extract genomic DNA for Illumina sequencing from vibriophages. Screening the literature I came across several methods for phage precipitation. I was wondering 1. which method is the...

27 March 2014 7,078 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View