Is a non-specific protein getting pulldown in my Co-IP experiment? If it is then how do I avoid this protein?

I am trying to pulldown a protein of 300 kDa using protein G agarose beads. I used two negative controls, one pulldown with just beads (without primary antibody) and another pulldown with another...

06 July 2019 5,760 9 View

Is there any database to know the change in protein expression before and after chemotherapy in patients?

I am looking for a database which provides information on the change in the expression of protein before and after chemotherapy. Any information in this regard would be appreciated.

11 December 2018 4,282 7 View

Can anyone provide information on how to maintain my animal cell culture?

We have been handling animal cell cultures for a long time. Recently, we are facing a problem which we never faced earlier. The problem is that the cell morphology looks pretty good, media is also...

08 September 2014 5,783 12 View

How to model a template protein that has two chains into a single chain target?

I have a protein which has two chains each contains 422 residues, both chains are identical. I want to model this protein but the target sequence which I have contains more than 850 residues. When...

07 August 2013 362 4 View

Is their any specific method apart from FACS for studying Rhodamine accumulation in the cell?

I want to monitor the accumulation of Rhodamine in cells. But I don't have the facility of FACS. So I wanted to know if there are any other alternative methods for monitoring accumulation of any...

03 April 2013 4,527 1 View

how a transformed cell proliferates with normal P53 activity?

as we all know that p53 will inhibit the cell to undergo cell division if the cell has carried some DNA mutation. how is it possible that a transformed cell which carries DNA mutation can still...

31 December 2011 5,750 28 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to manually calculate p value in ANOVA? what is the equation or formula for it?

How to manually calculate p value in ANOVA? what is the equation or formula for it?

03 March 2021 4,928 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View