I want to monitor the accumulation of Rhodamine in cells. But I don't have the facility of FACS. So I wanted to know if there are any other alternative methods for monitoring accumulation of any dye in cells.
Adam J Case
Safiulla,
Fluorescent confocal microscopy will probably be your best alternative. You could attempt to use a fluorescent plate reader, but you would need to rigorously wash the cells to make sure the dye was not in the media and truly in the cells.
Microscopy should be used whenever possible in conjunction with FACS to ensure the dye is truly inside the cells, and possibly not just associated with the membrane.
Good luck,
~Adam
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