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Questions related from Safiulla Basha Syed
I am trying to pulldown a protein of 300 kDa using protein G agarose beads. I used two negative controls, one pulldown with just beads (without primary antibody) and another pulldown with another...
07 July 2019 5,803 9 View
I am looking for a database which provides information on the change in the expression of protein before and after chemotherapy. Any information in this regard would be appreciated.
12 December 2018 4,343 7 View
I have been working on Cancer chemotherapy and Drug resistant cancer cell lines. Presently, I have developed two drug resistant cell lines. However, I need more cell lines to check the drug...
02 February 2016 7,411 2 View
We have been handling animal cell cultures for a long time. Recently, we are facing a problem which we never faced earlier. The problem is that the cell morphology looks pretty good, media is also...
09 September 2014 5,823 12 View
I have a protein which has two chains each contains 422 residues, both chains are identical. I want to model this protein but the target sequence which I have contains more than 850 residues. When...
08 August 2013 400 4 View
I want to monitor the accumulation of Rhodamine in cells. But I don't have the facility of FACS. So I wanted to know if there are any other alternative methods for monitoring accumulation of any...
04 April 2013 4,560 1 View
as we all know that p53 will inhibit the cell to undergo cell division if the cell has carried some DNA mutation. how is it possible that a transformed cell which carries DNA mutation can still...
01 January 2012 5,788 28 View
