What does a pan marker mean?

03 April 2015 1,316 5 View

Have you ever detected endothelial cells in bone marrow of rats , and if so, by which antibody?

Hi I am intending to detect endothelial cells, particularly sinusoidal endothelial cells, in bone marrow of rats. However, there are no much antibodies available for rats for that purpose. I was...

02 March 2015 4,057 7 View

Is it doable to double label two antigens of a cell with indirect fluorescence appraoch for one Ag, and biotin conjugated Ab for another Ag in FFPE?

Hi I am trying to do double labeling of endothelial cells in FFPE tissues by using a Biotin conjugated reagent against a surface marker, and an indirect fluorescence approach (primary Ab and...

02 March 2015 8,527 15 View

Do you know any protocol for doing immunohistochemical-paraffin embedded (IHC-P) double staining?

Hi I am trying to do IHC-P double staining for bone marrow tissues , but I am looking for a protocol for that purpose. I was wondering if you could help.

01 February 2015 5,615 2 View

Do you know any good source like ebook or website regarding the steps of maturation of all types of leukocytes in bone marrow?

Hi I am looking for a good source (ebook, website,etc) which clearly explains in which step of maturation all types of leukocytes live in bone marrow and in which step of maturation they leave the...

10 November 2014 3,998 0 View

Do you know any adipogenic inhibitor apart from BADGE?

I was wondering if you could name reagents which are routinely used to inhibit adipogenesis in animals ( in my case is rat)?

08 September 2014 1,191 3 View

Do you suggest any assay regarding (endothelial) cell proliferation apart from BrdU labelling?

Hi,I was wondering if you could suggest me any assay which deal with (endothelial) cell proliferation in vivo apart from BrdU labelling. I have been told that I may find other routine assays...

07 August 2014 9,421 16 View

How do you suggest to distinguish macrophages and monocytes in bone marrow by doing IHC?

Differences between monocytes and macrophages regarding their specific cell marker

05 June 2014 3,820 6 View

Extravasation of chemotherapeutic agents from blood vessels? Capillaries in particular?

I am looking for information regarding extravasation of chemotherapeutic agents from capillaries to tissues. If you could share your information, that would be very helpful.

02 March 2014 6,844 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View