Hi
I am intending to detect endothelial cells, particularly sinusoidal endothelial cells, in bone marrow of rats. However, there are no much antibodies available for rats for that purpose. I was wondering if anyone has done that before.
You might do de-calcified, paraffin-embedded, hematoxylin and eosin (H&E)-stained sections of bone marrow to localize the the endothelial cells (http://tpx.sagepub.com/content/34/5/548.full). I think flow cytometry might help.
Hi El rasheid
Thanks for your suggestion, but apart from H& E staining, I am planning to do immunohistochemistry staining of bone marrow to look at the sinusoidal endothelial cells and because of that I am seeking any antibody which may be helpful
CD31 is the universal marker. Also you will detect the bone marrow derived endothelial cells (there is such a thing too).
Decalcification in mild decalcifiers. I use Ostaosoft from Merck and is quite good for consecutive application of antibodies for IHC.
Anyway don't expect very strong signal and also expect variability in the IHC intensity.
Best & good luck,
Ivan
hi Ivan
thanks, but i could not find cd31anti rat antibody with application of IHC-P
http://www.abdserotec.com/rat-cd31-antibody-tld-3a12-mca1334g.html
This looks promising. And it is not so expensive if it fails.
Best,
Ivan
Hi
Here is another source of your antibody, rat anti-CD31
http://www.abcam.com/cd31-antibody-tld-3a12-ab64543.html
You could also consider to use VE-Cadherin as an endothelial marker if that works better for you.
good luck
Gopi
Hi Gopala
Thanks for your help.
But that Ab is not suitable for doing IHC for paraffin-embedded tissues. I found another company which have rat anti-CD31 with application of IHC-P. About the VE-cadherin, I have the same problem, I mean I could not find rat anti-Ve-cadherin with application of IHC-P. If I could, that would be really great.
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