What does a pan marker mean?

03 April 2015 1,316 5 View

Have you ever detected endothelial cells in bone marrow of rats , and if so, by which antibody?

Hi I am intending to detect endothelial cells, particularly sinusoidal endothelial cells, in bone marrow of rats. However, there are no much antibodies available for rats for that purpose. I was...

02 March 2015 4,057 7 View

Is it doable to double label two antigens of a cell with indirect fluorescence appraoch for one Ag, and biotin conjugated Ab for another Ag in FFPE?

Hi I am trying to do double labeling of endothelial cells in FFPE tissues by using a Biotin conjugated reagent against a surface marker, and an indirect fluorescence approach (primary Ab and...

02 March 2015 8,527 15 View

Do you know any protocol for doing immunohistochemical-paraffin embedded (IHC-P) double staining?

Hi I am trying to do IHC-P double staining for bone marrow tissues , but I am looking for a protocol for that purpose. I was wondering if you could help.

01 February 2015 5,615 2 View

Do you know any adipogenic inhibitor apart from BADGE?

I was wondering if you could name reagents which are routinely used to inhibit adipogenesis in animals ( in my case is rat)?

08 September 2014 1,191 3 View

Do you suggest any assay regarding (endothelial) cell proliferation apart from BrdU labelling?

Hi,I was wondering if you could suggest me any assay which deal with (endothelial) cell proliferation in vivo apart from BrdU labelling. I have been told that I may find other routine assays...

07 August 2014 9,421 16 View

How do you suggest to distinguish macrophages and monocytes in bone marrow by doing IHC?

Differences between monocytes and macrophages regarding their specific cell marker

05 June 2014 3,820 6 View

Can anyone help with immunohistochemistry of osteoblastic niche and identification of quiescent hematopoietic stem cells?

I was wondering if anyone could share how to identify osteoblastic niche for quiescent hematopoietic stem cells in bone marrow by doing IHC staining

02 March 2014 7,888 4 View

Extravasation of chemotherapeutic agents from blood vessels? Capillaries in particular?

I am looking for information regarding extravasation of chemotherapeutic agents from capillaries to tissues. If you could share your information, that would be very helpful.

02 March 2014 6,844 2 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Any suggestions for a website that allows you to create questionnaires and gather unlimited data (Totally free)?

I have found only four of them; www.surveyplanet.com www.online-anket.gen.tr www.surveey.com and of course google forms. Expecting to see your suggestions if there is any. Thank you

28 February 2021 3,237 3 View

Do I need to ask permission to use pictures for my questionnaire?

I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...

23 February 2021 1,631 3 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

How do you interpret multiple meta-regression in STATA?

Please could someone recommend a resource that explains performing meta-regression with multiple moderators. I have read the STATA META ANALYSIS REFERENCE MANUAL, but this only gives examples of...

17 February 2021 7,376 1 View

Do you know of an informal caregiver of a person who has recently received a bone marrow transplant?

We are conducting a pilot study of an intervention for informal caregivers of persons who have recently received a bone marrow transplant in the US. If you know of anyone who might be interested,...

13 February 2021 9,174 1 View

Does anybody know the ways of homogeneous ripening of Mung bean crop?

Some crops (Mung bean) are matured at different times i.e., harvested at several times that's why the labour cost of the farmer is high. So, could you please, is there any ways or chemicals are...

12 February 2021 294 4 View

Can anyone pls provide me information regarding the libraries where from i can get the absorption data of different materials used in solar cells??

I want to create .abs file ( absorption) for simulating solar cell in SCAPS. Can anyone pls provide me information regarding the libraries (websites) where from i can get the absorption data of...

10 February 2021 6,034 7 View