What does a pan marker mean?

03 April 2015 1,316 5 View

Have you ever detected endothelial cells in bone marrow of rats , and if so, by which antibody?

Hi I am intending to detect endothelial cells, particularly sinusoidal endothelial cells, in bone marrow of rats. However, there are no much antibodies available for rats for that purpose. I was...

02 March 2015 4,057 7 View

Is it doable to double label two antigens of a cell with indirect fluorescence appraoch for one Ag, and biotin conjugated Ab for another Ag in FFPE?

Hi I am trying to do double labeling of endothelial cells in FFPE tissues by using a Biotin conjugated reagent against a surface marker, and an indirect fluorescence approach (primary Ab and...

02 March 2015 8,527 15 View

Do you know any good source like ebook or website regarding the steps of maturation of all types of leukocytes in bone marrow?

Hi I am looking for a good source (ebook, website,etc) which clearly explains in which step of maturation all types of leukocytes live in bone marrow and in which step of maturation they leave the...

10 November 2014 3,998 0 View

Do you know any adipogenic inhibitor apart from BADGE?

I was wondering if you could name reagents which are routinely used to inhibit adipogenesis in animals ( in my case is rat)?

08 September 2014 1,191 3 View

Do you suggest any assay regarding (endothelial) cell proliferation apart from BrdU labelling?

Hi,I was wondering if you could suggest me any assay which deal with (endothelial) cell proliferation in vivo apart from BrdU labelling. I have been told that I may find other routine assays...

07 August 2014 9,421 16 View

How do you suggest to distinguish macrophages and monocytes in bone marrow by doing IHC?

Differences between monocytes and macrophages regarding their specific cell marker

05 June 2014 3,820 6 View

Can anyone help with immunohistochemistry of osteoblastic niche and identification of quiescent hematopoietic stem cells?

I was wondering if anyone could share how to identify osteoblastic niche for quiescent hematopoietic stem cells in bone marrow by doing IHC staining

02 March 2014 7,888 4 View

Extravasation of chemotherapeutic agents from blood vessels? Capillaries in particular?

I am looking for information regarding extravasation of chemotherapeutic agents from capillaries to tissues. If you could share your information, that would be very helpful.

02 March 2014 6,844 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View