Scientists showed that detection of monocytic differentiation in myeloid

neoplasms by immunohistochemical analysis is challenging owing to a lack of sensitive and/or specific antibodies, i wish these links help you.

Http://ajcp.ascpjournals.org/content/135/5/720.full.pdf+html

http://tpx.sagepub.com/content/40/2/345.full.pdf+html

At least in mouse f4/80 antibody recognizes macrophages.

Many thanks Esraa and Oscar for your help

Please consult Journal of Fish Diseases 2006, 29, 43–48 E S Munro et al. In infectious pancreatic necrosis virus carrier Atlantic

salmon, Salmo salar L., post-smolts, almost all kidney

macrophages ex vivo contain a low level of non-replicating

virus.

In humans, monocytes will express CD14 while macrophages will not. In IHC, you'll have the further advantages of them having a distinct morphology.

Good luck

G

Thanks Grant.

What about in Rats and Mice? Do you suggest any distict marker?