We are having some problems culturing OPCs from the C57BL6 strain compared to the CD1 one. The protocol is as usual: we obtain OPCs from P0s mice brains, culture them in flasks in a mixed culture with DMEM supplemented with serum (FBS), shake them overnight and then seeded in a P96 plate. Does anybody have any clue about different media, serums, time of shaking? Thank you in advance.
Bangfu Zhu
For mouse OPC culture, it's better to use OPC medium (DMEM with SATO and Insulin/Transferrin) and coat the culture plates with PDL and Laminin. Good luck!
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