Carolina Melero-Jerez

11 Questions 13 Answers 0 Followers

Questions related from Carolina Melero-Jerez

How can I deplete IFNß specific T cell response?

I would like to separate the in vivo IFN-ß effects observed in the EAE model attending to different subsets. For that purpose, I would need to specifically deplete the intracellular pathway for...

07 July 2017 1,749 6 View

How could we eliminate astrocytes from a mixed glial cell culture?

We are trying to obtain a pure oligodendrocyte culture from p0 mice. After one week of mixed glial culture in ornitine-coated flasks, we purified the oligodendrocyte precursor cells (OPCs) by...

03 March 2016 4,470 9 View

Which medium is the best for ATCC A2B5 hybridome culture?

Hi everyone! I am trying to culture cells from ATCC (ref: CRL-1520-ATCC) in order to obtain the A2B5 antibody. As the company recommends, I use a DMEM formulation from Sigma-Aldrich (D-7777) with...

01 January 2016 9,215 4 View

Can we freeze the trypan blue solution?

We wonder if the freezing may affect to the properties of the trypan blue. Thank you in advance!

12 December 2015 4,362 5 View

Any reason for the absence of policlonal activation of CD3+ T-cells in culture?

Hi everybody, I've been growing T cells from a flow cytometry CD3-sorting for a long time, obtained from C57BL6 mice spleens. At least three hours before seeding, the p96-well plate is coated...

06 June 2015 8,905 7 View

Any good Foxp3 antibody for IHC in spinal cord?

Does anybody know a good antibody for the detection of T regs in spinal cord? The IHC would combine CD4/CD8, CD25 and Foxp3. Thank you in advance.

03 March 2015 2,629 3 View

Does anybody have a protocol for freezing MDSCs?

I would like to freeze MDSCs obtained after a FACS sorting. Has anybody a specific protocol for doing that? Or should I use just fetal bovin serum with DMSO? Thank you in advance.

11 November 2014 9,776 3 View

How can we effectively detach aCD3/aCD28 stimulated T lymphocytes from the p96 wells?

We are stimulating T lymphocytes using a coating of aCD3 and aCD28 on p96 wells, but we have difficulties to detach them after 24 hours. Does anybody have an idea of how to achieve that? We need...

09 September 2014 1,771 8 View

Can anyone help with C57 mice OPCs culture?

We are having some problems culturing OPCs from the C57BL6 strain compared to the CD1 one. The protocol is as usual: we obtain OPCs from P0s mice brains, culture them in flasks in a mixed culture...

08 August 2014 4,219 1 View

Can anyone help with a coating suggestion for T-cell stimulation?

After sorting the CD3 positive cells from the spleen, I stimulated them in a p96 previously coated with anti-CD3 and anti-CD28 for 3 hours, and then I cleaned the wells with PBS. Does anybody...

05 May 2014 4,215 5 View

Does CD8 fluorescence intensity decrease in a stimulated CD3 positive cells culture?

We want to measure the level of activation of the different populations of CD3 positive cells after a CD3 sorting (using FACS Aria, CD3-PB). We stimulate the cells obtained from the sorting with a...

12 December 2013 5,598 3 View