I run real time sybr green pcr (rat hippocampus) and I came accros a primer dimer situation. I use 42.5 nM primers in final concentration. I am getting PDs in whole range of concentrations, from 10 ng to 0.001 ng. I can attach my primer sequences if needed.
5 HT1a (F) – gtcctgcctttctgtgaaagca
5 HT1a (R)- tatggcacccaacaacgca
p11 (F) – tgctcatggaaagggagttc
p11 (R)- ccccgccactagtgatagaa
β-arrestin (F) – aagacaccaacctggcttcc
β-arrestin (R) – taggacgaaaggtagctccac