I run real time sybr green pcr (rat hippocampus) and I came accros a primer dimer situation. I use 42.5 nM primers in final concentration. I am getting PDs in whole range of concentrations, from 10 ng to 0.001 ng. I can attach my primer sequences if needed.

5 HT1a (F) – gtcctgcctttctgtgaaagca

5 HT1a (R)- tatggcacccaacaacgca

 

p11 (F) – tgctcatggaaagggagttc

p11 (R)- ccccgccactagtgatagaa

 

β-arrestin  (F) –  aagacaccaacctggcttcc

β-arrestin  (R) – taggacgaaaggtagctccac

More Milos Mitic's questions See All
Similar questions and discussions