Yes you can get to callus growth. This method called suspension culture. This method gave fragile callus. Callus in this method gave embryonic callus. This is best method to production of somatic embryos from callus culture.
May be easy absorption of nutrients from liquid medium compared with the solid medium
Its better and the best you can use shaker with 50 RPM with little of 2,4-D concentration (0.1- 0.5 mg/l) to get friable callus for embryogenesis induction. this is for general concept.
what if the callus is hard and if i inoculate it into liquid media directly? what will be the result?
Actually this is will depend on auxin type, 2,4-D will give you yellow friable callus then embryo, TDZ will give you more compact callus with green color, NAA will give you more white compact callus. But before that you should make sure that your plant has already have ability to embryo induction.
i have used 2,4-D and it gave hard callus on solid media so if i inoculate it into liquid media will i get the result.
Try to collect fresh callus by peeling hard callus ( about 4-5 mm size) and inoculate into liquid media on shaker. did you check ability of embryo induction of your plants in previous researches? what is the plant that you work on?because many plants has easy ability for callus induction but without ability of embryo induction.
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