Components used:

DMEM Powder – 1 pack

Glucose- 4.5gm/ L

Sodium bicarbonate – 3.7gm/L

MilliQ water- 1L

PROCEDURE:

1. The entire powder in the pack was dissolved in 900ml of milliQ water.

2. 4.5 gm of glucose and 3.7gm of sodium bicarbonate were added.

3. The pH was adjusted to 6.9 with HCl.

4. The media was made up to 1000ml with milliQ water.

5. After the components were completely dissolved, the medium was filtered and stored.

Components used:

DMEM Powder – 1 pack

Glucose- 4.5gm/ L

Sodium bicarbonate – 3.7gm/L

MilliQ water- 1L

PROCEDURE:

1. The entire powder in the pack was dissolved in 900ml of milliQ water.

2. 4.5 gm of glucose and 3.7gm of sodium bicarbonate were added.

3. The pH was adjusted to 6.9 with HCl.

4. The media was made up to 1000ml with milliQ water.

5. After the components were completely dissolved, the medium was filtered and stored.

I hope the following links may help you

http://himedialabs.com/TD/AT068.pdf

p://www.sigmaaldrich.com/life-science/cell-culture/classical-media-salts/dmem.html 

http://himedialabs.com/TD/AT068.pdf

Thank you madam