Can any one give any simple protocol for annexin v pi staining for cell line flow cytometry analysis with or with out cell fixation ?

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3T3-L1 cells are growing slow in The DMEM/10%FBS media; any reason for that ?

3T3-L1 adipocytes

31 December 2018 3,313 4 View

Any place to Buy Hep3B cell line other than NCCS pune In India ?

Hep3B cell line

07 August 2017 6,380 0 View

Please do suggest any Liver control cell line For liver cancer ?

Liver control cell line liver cancer

01 February 2017 2,293 5 View

Hello Dear Scientists Please explain use of Chang liver cell line why we use? can we use for anticancer activity ?

Chang liver cell line why we use? can we use for anticancer activity ?

01 February 2017 858 0 View

Can any body tell about standard drug concentration for Cytotoxicity assay ?

Cytotoxicity cell lines

07 August 2016 2,872 2 View

Can i use "chang liver" cell line as control cell line for cancer cell line?

control cell

07 August 2016 1,795 0 View

Dear Friends Please help me to do autodocking in Mac OS, what are the sofware need and any installation and working procedure for that?

autodocking in Mac OS, Softwares in autodocking

06 July 2016 6,565 0 View

How to do dapi staining for Adherent cells? its same to PI staining for cell cycle assay please clarify ?

daps staining PI staining for cell cycle assay

06 July 2016 5,720 4 View

Can i use 5-fluorouracil as a control Drug for liver cancer cell lines ?

control Drug for liver cancer cell lines

06 July 2016 1,303 2 View

Can anyone tell me what is suitable control cell line for the liver cancer cell line HepG2 ?

control cell line for HepG2 cell line

04 May 2016 2,301 0 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View