11 Questions 16 Answers 0 Followers
Questions related from Nimal Raveendran
I am using some primary human cells for my experiments, But the activity/proliferation rate of the cells in their late passages (>P8) are very low and I can not use them for long like cell...
12 December 2017 4,881 5 View
(a) What is the rationale of doing a slow freezing (controlled rate freezing)? (b) Why a quick thawing of frozen vials at 37 degree C? I read some research articles , but confused about...
12 December 2017 7,871 8 View
I want to study the effect of a hormone in stem cells. I know the dose (20 mg in 80 microL) used for I.V injection. How can I determined the dose that I have to use for cell culture studies? Can I...
02 February 2016 6,341 0 View
What is the relevance of studying morphology of an injectable gel? Can we visualize the porous structure of injectable gels by SEM imaging of freeze dried samples ? If not can you suggest me some...
05 May 2015 2,686 5 View
I am searching for a protocol to isolate fibrin from bovine blood.
11 November 2013 924 2 View
Usually we keep extract overnight after adding 1/10th volume of sodium acetate and 2 volumes of ethanol. My question is, if we keep it at -20 Celsius for 3 days, will it affect DNA quality? We are...
10 October 2013 1,098 15 View
During my PG, in our university we had both life science and Biotechnology department. But both departments were teaching same subjects,doing similar research. I am confused between these two...
09 September 2013 7,917 12 View
Using 'Highlight sequence features' option in NCBI, we have retrieved all CDS of a full genome. From this data set, CDS having less than 300 nucleotide were excluded. Now how should we proceed to...
09 September 2013 3,764 3 View
What are the differences of keratin molecule isolated from human hair and chicken feather in terms of structure and properties?
09 September 2013 4,923 1 View
We want to isolate genomic DNA from plant leaves by using CTAB method. Unfortunately we don't have PVP. Can we use other chemicals instead of PVP to absorb polyphenols? How can we modify the...
09 September 2013 7,131 5 View
In our lab we isolated the extracellular matrix from human adipose tissue and digested using 0.5M acetic acid. When I mixed the digested ECM with SDS loading dye (contains SDS, Bromophenol blue,...
08 August 2013 6,209 2 View
