3T3-L1 cells are growing slow in The DMEM/10%FBS media; any reason for that ?

More Sylvester Darvin Sandhaanam's questions See All

Can any one give any simple protocol for annexin v pi staining for cell line flow cytometry analysis with or with out cell fixation ?

annexin v staining protocol adherent cells with cell fixation for flow flow cytometry

09 October 2017 499 2 View

Any place to Buy Hep3B cell line other than NCCS pune In India ?

Hep3B cell line

07 August 2017 6,380 0 View

Please do suggest any Liver control cell line For liver cancer ?

Liver control cell line liver cancer

01 February 2017 2,293 5 View

Hello Dear Scientists Please explain use of Chang liver cell line why we use? can we use for anticancer activity ?

Chang liver cell line why we use? can we use for anticancer activity ?

01 February 2017 858 0 View

Can any body tell about standard drug concentration for Cytotoxicity assay ?

Cytotoxicity cell lines

07 August 2016 2,872 2 View

Can i use "chang liver" cell line as control cell line for cancer cell line?

control cell

07 August 2016 1,795 0 View

Dear Friends Please help me to do autodocking in Mac OS, what are the sofware need and any installation and working procedure for that?

autodocking in Mac OS, Softwares in autodocking

06 July 2016 6,565 0 View

How to do dapi staining for Adherent cells? its same to PI staining for cell cycle assay please clarify ?

daps staining PI staining for cell cycle assay

06 July 2016 5,720 4 View

Can i use 5-fluorouracil as a control Drug for liver cancer cell lines ?

control Drug for liver cancer cell lines

06 July 2016 1,303 2 View

Can anyone tell me what is suitable control cell line for the liver cancer cell line HepG2 ?

control cell line for HepG2 cell line

04 May 2016 2,301 0 View

What is the reason for current dropping in OER , LSV curve?

I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...

10 August 2024 3,629 1 View

What may be the reasons for failures of Tube toi Tube Sheet Joints in Boiler Drum ?

We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.

08 August 2024 3,161 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

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CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

Nelma Gina

We use 10% NBCS in our lab instead of 10% FBS. That is unless we want to differentiate the pre-adipocytes into adipocyte-like cells, then we use FBS.

Sylvester Darvin Sandhaanam

Thank you. we also wanted to differentiate the pre-adipocytes into adipocyte-like cells that's why I am asking

Matías Gabrielli

Please, check that your DMEM has "High Glucose" content (4500 mg/l).

Thank you