Can any one give any simple protocol for annexin v pi staining for cell line flow cytometry analysis with or with out cell fixation ?

annexin v staining protocol adherent cells with cell fixation for flow flow cytometry

09 October 2017 393 2 View

Any place to Buy Hep3B cell line other than NCCS pune In India ?

Hep3B cell line

07 August 2017 6,272 0 View

Hello Dear Scientists Please explain use of Chang liver cell line why we use? can we use for anticancer activity ?

Chang liver cell line why we use? can we use for anticancer activity ?

01 February 2017 763 0 View

Please do suggest any Liver control cell line For liver cancer ?

01 February 2017 2,201 5 View

Can any body tell about standard drug concentration for Cytotoxicity assay ?

07 August 2016 2,775 2 View

Can i use "chang liver" cell line as control cell line for cancer cell line?

07 August 2016 1,674 0 View

Dear Friends Please help me to do autodocking in Mac OS, what are the sofware need and any installation and working procedure for that?

autodocking in Mac OS, Softwares in autodocking

06 July 2016 6,466 0 View

How to do dapi staining for Adherent cells? its same to PI staining for cell cycle assay please clarify ?

06 July 2016 5,619 4 View

Can i use 5-fluorouracil as a control Drug for liver cancer cell lines ?

control Drug for liver cancer cell lines

06 July 2016 1,201 2 View

Can anyone tell me what is suitable control cell line for the liver cancer cell line HepG2 ?

04 May 2016 2,204 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View