I want to transfect mirna mimic /inhibitor to primary B cells,Can any one provide the protocol for Nucleofection of mirvana mimic ,inhibitor via nucleofector4 D X
Can any one provide a transfection protocol for MIRVANA mirna mimic /inhibitor?
Hi, For compensation, we can use single-stained beads in flow cytometry. For gating control or FMO: can we just mix single-stained beads to get FMO?
25 May 2024 6,832 1 View
In 84232 add gene plasmid, I am not sure at which site should I clone guide RNA
04 April 2024 8,044 3 View
In addgene 84323, plasmid they have empty gRNA casette. I am not sure where to clin guide RNA of interest. 1. Is it should be between U6 promoter and gRNA casette or 2. In gRNA casette itself?
04 April 2024 8,157 3 View
I have been using the traditional method for characterizing microbial fuel cell where at the end we will obtain power density curve (Power density vs Current) and polarization curve ( Voltage vs...
14 February 2024 957 0 View
I am doing a GC MS analysis of plant volatile compounds. I am observing that the volatile concentration of few compounds decrease in presence of sodalime. Can somebody let me know if soda lime...
09 June 2019 1,712 6 View
I am not clear what is rule behind mirna and mRNA targeting pair,because each mirna target officially 100s of target. In such cases how one will validate legitimate mirna mRNA target. Assays...
21 April 2019 420 4 View
This question is related to soluble film formulation.
04 September 2018 3,223 1 View
PET labelled forward primer for microsatellite detection along with target region, PCR artifact shadow peak of equal intensity observed. artifact since it is not visible on agarose gel.
10 March 2018 2,867 3 View
I want to know how to design primer for primary miRNA QRT PCR
05 March 2018 4,991 6 View
How to draw Scatchard plot, using data of fluorescence intensity and concentrations of ligands and protein only. Please help..
22 January 2018 1,232 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View