How mirna chose its target among 100s of mRNA having seed sequence?

I am not clear what is rule behind mirna and mRNA targeting pair,because each mirna target officially 100s of target. In such cases how one will validate legitimate mirna mRNA target. Assays...

21 April 2019 281 4 View

Films prepared by combination of Hypromellose E15 and E5 polymer with 20% of plasticizer (PEG 400) w.r.t dry polymer using water results in brittle ?

This question is related to soluble film formulation.

04 September 2018 3,086 1 View

How to meassure primary miRNA trancript using QRT PCR?

I want to know how to design primer for primary miRNA QRT PCR

05 March 2018 4,842 6 View

How to draw Scatchard plot, using data of fluorescence intensity and concentrations of ligands and protein only?

How to draw Scatchard plot, using data of fluorescence intensity and concentrations of ligands and protein only. Please help..

22 January 2018 992 4 View

I wanted to see if all the morphs of nematodes are same species or not by molecular sequencing, how to proceed doing it?

20 March 2017 5,885 3 View

What is the function of acetic acid and methanol in staining-destaining of sds page?

function of acetic acid and methanol in staining-destaining of sds page

17 February 2017 3,392 4 View

Can miRNA mimics or inhibitor used in primary cells?

I am currently working with lymphocyte ,I want to know if any one has used the miRNA mimics or inhibitor with primery cells

02 November 2016 1,753 3 View

How to reduce size of a void in a metal from micron to nanometer range ??

I want to know if there is a way in which the void of the material inside the metal can be divided from micron size to nanometer size by splitting the voids into discrete voids of reduced size...

03 October 2016 6,811 3 View

How is the molecular phylogenetic analysis of foraminifera beneficial for taxonomic studies?

I would like to know the actual role of rDNA and protein sequences in evolutionary relationships of foraminifera.

23 August 2016 9,210 2 View

Has anyone implemented Self Organizing (Adaptive) Fuzzy controller?

I have read a lot theory about Self Organizing Fuzzy control from Internet. But I am still not completely able to understand its theory and implementation.I have attached one of the best document...

02 June 2016 2,438 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View