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Questions related from Nicholas Samuel Brown
I did 16S PCR and Sanger sequencing to see if the expected bacteria were present in my co-culture experiments. I did a nucleotide BLAST under the refseq_genomic database for highly similar...
01 August 2017 6,936 5 View
I want to use the SpeedVac concentrator to concentrate some gDNA samples i have extracted from a co-culture experiment. The samples contain both prokaryotic and eukaryotic DNA. Eventually only the...
25 July 2017 6,719 2 View
I run a co-culture experiment with HeLa (mammalian) cells and bacteria (P. bivia, P. disiens, L. crispatus). Following a co-incubation period i want to measure the viability of the HeLa cells...
24 July 2017 6,543 3 View
I am running a co-culture experiment with HeLa (mammalian cells) and various bacteria (P. disiens, P. bivia, L. crispatus). I need to measure the viability of the HeLa cells following a...
19 July 2017 1,149 1 View
I always understood fold change calculations following a qPCR to be calculated by 2^-(ddCT). Is this incorrect, my mentor thinks it is? Potentially they are thinking of something else. Could you...
10 July 2017 2,377 5 View
Currently I am growing Lactobacillus crispatus, Prevotella disiens, and Prevotella bivia in purely anaerobic conditions. I was wondering if anyone knew if Prevotella species are viable in...
02 May 2017 9,451 1 View
First of all what does the effect size reported for different eQTL's represent in the GTEX database? Furthermore, what does a negative and positive effect size mean? Im exploring eQTL's in whole...
10 April 2017 9,227 1 View
Does anyone know if Calcein AM assays have been successfully used on anaerobic cells to measure cell viability/ cell proliferation? A link to literature supporting your answer would be greatly...
03 April 2017 5,499 0 View
Does anyone know sequences for an 18S primer? The primer would be used as a housekeeping gene. I am working with HeLa cells to measure gene expression
15 March 2017 9,218 3 View
I've been given a large list of entrezID/Gene ID from NCBI (example:145864). Is there anyway to fetch chromosome number and nucleotide range from NCBI gene database...
03 March 2017 2,119 1 View
I have been given a chromosome number and range (example:chr1:20369369-20490791) and I need to be able to find out if this range falls within another chromosomal range and if it does not how far...
03 March 2017 6,332 2 View
I've been given a large amount (~2500) of ensemble numbers (example:ENSG00000175445) for various genes how can I quickly find the chromosomes they are located on, and the exact section range they...
01 March 2017 6,892 0 View
