How does a Bi-Static FMCW radar detect a Zero Doppler target?

27 September 2020 8,370 6 View

What is the best way to extract phenols from plants ?

What is the best way to extract phenols from plants ?What is the best way to extract phenols from plants without any loss

27 May 2020 9,005 17 View

Problem on reliability statistics - negative Cronbach's alpha?

In my study I want to see whether 3 items in a questionnaire relate to autonomy through a reliability analysis. The following three items are relevant I think: - Availability of task enrichment...

25 July 2018 3,223 2 View

What techniques can be used to locate fractures in underground metal water delivery pipes?

I recently met with an senior executive of a Water delivery company. #1 concern was the inability to locate leakage in underground mains pipes. Typically 40% of water is lost between source and...

24 May 2018 4,311 3 View

Would you like to participate in studies involving surgical outcomes, NSQIP, NIS, SEER, etc?

Seeking co-authors/collaborators to participate with me in database studies involving NSQIP, NIS, SEER, NAMC, etc. This is a good opportunity for post-docs and research fellows who can work...

04 November 2017 6,424 2 View

I am using simply P total RNA extraction kit (Bioflux, china) to extract total RNA from tissue sample do it require addition of mercaptoethanol

Does it require addition of lysis buffer or mercaptoethanol before homogenization or it's included in components of the kit reagents?!

03 August 2017 2,599 4 View

How many kinds of distance defined on graph in graph theory?

04 April 2017 4,384 13 View

How can I dissolve sodium palmitates in pure ethanol?

18 June 2016 784 3 View

Cell size units in point to raster?

I have metadata in my .dat file that tells me that my cell size is 0.200 degrees longitude. I want to import this file into GIS and convert my points to raster. My file is very simple: it has x, y...

29 September 2015 7,692 3 View

How can we assess the amount of sand available in a river and a lake?

What are the measures to be taken for controlled sand mining. Excess sand mining has become a big problem due to rapid urbanisation and development. Uncontrolled sand mining has now resulted in...

30 January 2014 5,444 1 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Does soft algniate hydrogel influence calcium concentration of a solution (cell culture medium)?

05 January 2021 6,066 4 View

How to prepare 2mM phosphate buffer as Na2HPO4 and K2HPO4 added in a 4:1?

I'm trying to do an Intracellular Ca2+ mobilization assay and I need to add phosphate buffer as Na2HPO4 and K2HPO4 added in a 4:1? should I prepare 2mM of Na2HPO4 and 2mM of K2HPO4 separately then...

05 October 2020 5,584 2 View

How to simulate a 5M NaCl solvated protein with two Ca2+ ligands in GROMACS properly?

Hi, I'm kinda new to computational biochemistry. so here are some few questions of mine: I made an amylase system solvated with NaCl 5M via PACKMOL, and the pdb file of solvated amylase was...

09 August 2020 2,490 2 View

How can I calculate the composition and quantity of different types of calcium aluminates and sulphides from EDS analysis of inclusions in steel?

How can I calculate the composition and quantity of different types of calcium aluminate (CA6, CA2, CA,...) and sulphides (CaS, MnS) from the EDS analysis result of inclusions present in Al-killed...

07 August 2020 4,621 1 View

How can we check the real content of calcium in the protein samples?

03 August 2020 4,309 3 View

Does C18 desalting column can be used to remove amino acids and ions?

Hi there, I want to remove any ions (mostly Ca2+, amino acids) from my soluble protein extract preparation. I came across with idea of using C18 column which only binds to peptides and rest will...

25 July 2020 9,931 3 View

Why does the adsorption ability of a zeolite decrease if there is an increase in temperature?

Hello, so I'm doing some research on clinoptilolite, a naturally made zeolite. I'm testing on its ability to treat hard water (water with high levels of magnesium/calcium), and how temperature can...

25 April 2020 8,679 9 View

I'm obtaining some variable data from an intacellellular Ca2+ influx assay using Fura-2AM and require some troubleshooting help, can anyone assist?

-I am using Fura-2AM dye with probenecid to permiabilise TR146 oral epethelial cells. -flourescence is detected on a Flexstation. -Results from current experiments vary significaltly to previously...

29 March 2020 8,088 4 View

Can addition of GABA agonist block transients in neurons(See description for details)?

I am trying to characterise GABA developmental switch in iPSC derived neurons using Ca2+ imaging. However, as I add GABA agonist, I observe the spontaneous activity of several neurons being...

08 September 2019 694 2 View