Amera A.M Abdilnabi

12 Questions 8 Answers 0 Followers

Questions related from Amera A.M Abdilnabi

Can i use probenecid (water insoluble) in measuring intracellular calcium concentration from HIT_T15 cells?

Dear Researcher, I am working on HIT_T 15 cells and I want to measure the intracellular calcium concentration using Fura_2am, I found many protocols using probenecid , My question: is it ok if i...

18 April 2023 6,481 0 View

Can I transfare the cells from well to another well?

I made a mistake, I seeded my cells in 6 well plates instead of 96 well plates. my question is can I transfer my cells to 96 well by using trypsin is that possible?

13 February 2023 7,854 0 View

How do I prepared 0.25% (w/v) Trypsin 0.53 mM EDTA solution?

The molecular weight of EDTA is 292.24g/mol Thanks in advance!

02 February 2023 5,462 3 View

What is the proper protocol for [Ca2+]! concentration measurement ?

I'm working on peptides from frog skin secretions, and I need to measure the concentration of [Ca2+]! release from HIT-T15 cells. I'm confused about the proper protocol for [Ca2+]! assay by using...

13 May 2022 817 1 View

Should I make a different dilution for my samples in BCA assay ?

This is my first time doing a BCA assay for my protein sample, So my question is should I make a serial different dilutions for my sample or make dilutions with the same concentration? as the...

29 October 2021 4,783 2 View

protocol for ca+2 concentration measurement in HIT-T15 cells using Fura-2Am?

Hi everyone,Id like to ask if anyone had a protocol for ca+2 concentration measurement in HIT-T15 cells using Fura-2Am.Because I cannot find any suitable one.

07 July 2021 2,579 0 View

Type of medium used for MTT assay ??

I’m working on HIT_T15 cells , and I would ask about MTT assay!! i Know that the medium should be without phenol red , but most of the mediums I found it available was without phanol red and...

27 May 2021 8,401 3 View

Protien elution using sep pak ?

Hi everyone, I’d asking about the elution step of protien in sep pak c18 , this is the first time that I’ve made the elution with acetonitrile , the question is when the elution process done the...

24 May 2021 8,757 6 View

Connect c18 column in AKTA-pure through unicorn software

Hello dear researchers and experts, Could anyone help me regarding the C18 column? I want to do protein purification using AKTA-pure, so when I insert the parameters in unicorn software I didn't...

01 January 1970 4,497 0 View

[Ca2+]! concentration measurement( calcium release from HIT-T15 cells)

Hello everyone. I'm working on peptides from frog skin secretions, and I need to measure the concentration of [Ca2+]! release from HIT-T15 cells. I'm confusing about the proper protocol for...

01 January 1970 8,798 0 View

Akta pure Chromatogram from AKTA-pure (Protein purification)

Hello esteemed researcher, May I direct your attention to the image? I am curious to understand why there is only a minimal drop in pressure once the elution begins. thank you.

01 January 1970 2,789 5 View

MTT assay , HIT_T15 cells