Amera A.M Abdilnabi

6 Questions 6 Answers 0 Followers

Questions related from Amera A.M Abdilnabi

Can I transfare the cells from well to another well?

I made a mistake, I seeded my cells in 6 well plates instead of 96 well plates. my question is can I transfer my cells to 96 well by using trypsin is that possible?

13 February 2023 7,715 0 View

How do I prepared 0.25% (w/v) Trypsin 0.53 mM EDTA solution?

The molecular weight of EDTA is 292.24g/mol Thanks in advance!

02 February 2023 5,396 3 View

Should I make a different dilution for my samples in BCA assay ?

This is my first time doing a BCA assay for my protein sample, So my question is should I make a serial different dilutions for my sample or make dilutions with the same concentration? as the...

29 October 2021 4,714 2 View

protocol for ca+2 concentration measurement in HIT-T15 cells using Fura-2Am?

Hi everyone,Id like to ask if anyone had a protocol for ca+2 concentration measurement in HIT-T15 cells using Fura-2Am.Because I cannot find any suitable one.

07 July 2021 2,517 0 View

Protien elution using sep pak ?

Hi everyone, I’d asking about the elution step of protien in sep pak c18 , this is the first time that I’ve made the elution with acetonitrile , the question is when the elution process done the...

24 May 2021 8,685 6 View

Connect c18 column in AKTA-pure through unicorn software

Hello dear researchers and experts, Could anyone help me regarding the C18 column? I want to do protein purification using AKTA-pure, so when I insert the parameters in unicorn software I didn't...

01 January 1970 4,435 0 View