I'm currently working on my Master's project on A.baumannii phages and I'm looking for some factors that might increase phage titer. I've checked the Ca2+ and Mg2+ supplements, which worked well depending on the specific phage. Also, i've checked shaking, different MOI and pH. What else can i check?
Some antibiotics are thought to stimulate bacteria to produce phages; perhaps you should try adding antibiotics to the culture media.
If your bacterial host is resistant, you can use the standard laboratory concentration, or you could also use subinhibitory concentrations.
It depends on your protocol as well, are you doing liquid cultures or plates cultures? But often the most important factor is the MOI, changing that can give large differences in final titer.
Lastly, it also depends upon the phage itself and its burst size, so some phages will just always give higher titers than other phages.
Michael J. Benedik Yes! Indeed MOI and shaking worked the best so far, but the outcome also depends on in-between phages. I'm currently amplifying phages in liquid culture, not on plates
Of course each phage will be different. In general you will get higher titers on plates but you are volume limited, so for larger volumes you need to go with liquid.
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