Hello!
If I have an alginate gel cross-linked with 20mM Ca2+, how can I add and remove culture media without washing away the Ca2+ from the alginate?
How much Ca2+ should I supplement my media with?
The attached file demonstrates that there is a predictable relationship between the structural density of a terrestrial body and its none-rotating surface gravity. Using Newton's gravity law of...
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From the way we define the phi and Cramers V and from my theoretical knowledge, I expect Phi coefficient and Cramer's V to bare positive values. In a recent analysis using SPSS 22.0, Negative...
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After CO2 Electrochemical reduction on 6 samples, i obtained both gaseous and liquid products from GC-MS and NMR respectively. i quantified the liquid products by integrating each peak. for...
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I looking for ferro calcium production process
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Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...
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Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...
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Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...
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I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...
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I am currently working on a book on Public Relations (from an African perspective) and the rationale behind the book is the fact that as instructors in this field, we borrow instances and case...
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I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...
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I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...
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Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...
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