The molecular weight of EDTA is 292.24g/mol
Thanks in advance!
Hello Amera A.M Abdilnabi
You may first want to prepare 100ml of 0.53mM EDTA solution.
The molecular of EDTA is 292.24g/mol.
292.24g – 1 L – 1M
0.0154g - 100ml – 0.53mM
So, weigh 15.4mg EDTA and prepare 100ml of EDTA solution (0.53mM) in MilliQ water.
For 0.25%(w/v) Trypsin, you need to weigh 250mg Trypsin and add to the 100ml EDTA solution (0.53mM) which you have prepared above.
Please note: EDTA is insoluble in water. So you may have to use EDTA Disodium salt, which is a soluble salt. The Mol wt. of EDTA Disodium salt is 372.24 g/mol.
So, prepare 0.25% (w/v) Trypsin 0.53 mM EDTA solution by referring to the above protocol, but using EDTA Disodium salt with Mol.Wt. 372.24 g/mol.
Best.
Malcolm Nobre Thank you so much for your help.
So if I use EDTA with M.W 372.24g/mol
weight 197mg of EDTA in 100 ml water, am I right?
Amera A.M Abdilnabi you should weigh 0.0197g of EDTA Disodium salt in 100 ml MilliQ water. So it should be 19.7mg and not 197mg of EDTA Disodium salt.
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