I'm working on peptides from frog skin secretions, and I need to measure the concentration of [Ca2+]! release from HIT-T15 cells. I'm confused about the proper protocol for [Ca2+]! assay by using Fura-2 Am, as I found many unclear protocols.

please could anyone guide me in this matter, and give me the right and clear protocol.

I really appreciate any help you can provide.

More Amera A.M Abdilnabi's questions See All
Similar questions and discussions