How can I determine the appropriate concentration of Ca2+ chelator (BAPTA-AM) to pre-treat plants (rice) for studying intracellular Ca2+ signaling?

Kokila Wickramanayake @Kokila-Wickramanayake

06 December 2023 0 7K Report

I am interested in mapping the concentration of intracellular Ca2+ in rice plant cells. As a control treatment, I plan to apply the Ca2+ chelator BAPTA-AM. If anyone here has experience using Ca2+ chelator to reduce intracellular Ca2+ in rice or plants, could you please suggest the appropriate concentration of the chelator to use and provide any protocol suggestions? Thank you.

Badges
Science topic

More Kokila Wickramanayake's questions See All

Can someone help me by sending a sample lot of Coarse Aggregates that consists of reactive silica?

I am doing a research on Alkali silica reaction in concrete. For that I want to collect coarse aggregates that consists of reactive silica that leads alakali-silica reaction (ASR) in concrete. To...

15 February 2023 9,648 0 View

Any researcher who could give some insights regarding what kind of Hydroxyl Irons (OH-) do react with Silica resulting Alkali Silica Gel in Concrete?

It is about ASR in concrete.

26 July 2022 6,661 0 View

Can someone recommend a Recycled Concrete Aggregate producing plant/facility that can be visited/observed in academic point of view out of Sri Lanka?

I expect to visit such a recycling plant to gain knowledge on the concrete recycling process. I have visited one and only plant in Sri Lanka and seeking to visit a few in international context.

19 July 2022 9,169 0 View

How to solve Autogrid error during Autodock?

I am using PyRx as platform when I load macromolecule and ligand, the molecules get loaded. When I Dock I get this message " >>Python 2.6.5 (r265:79096, Mar 19 2010, 21:48:26)" Can anyone...

02 May 2022 5,815 4 View

Can secondary metabolites production be expected in plant callus culture, if the callus culture isn't entered into the regeneration stage?

Tring to develop an alternative source to extract bioactive compounds from endangered medicinal plants. Callus cultures will be one of the options. But having a doubt of whether the...

05 February 2022 4,863 3 View

What is the best method to take photos of Plant Tissue Cultures and how to add size bar ?

I want to make photographs of my plant tissue cultures for publications.

20 September 2021 1,986 10 View

Why there is drop in OD in culture of bacillus subtilis observed in fermenter?

OD REACHES 27-30 then it suddenly crash down to 7od. It never behaved like this. I just need to know if anyone experienced the same or does anyone know the answer.

18 May 2016 6,408 2 View

How to draw the five chelate rings of [Co(EDTA)]- on this picture?

Is this circle one of the rings?

03 August 2024 5,988 0 View

What is homomenthyl cinnamate?

the molecule name is citated in old literature about organic sunscreen molecules

10 July 2024 5,576 2 View

What causes unexplained rash on the whole body after consuming Stillnox and Serdep in patients on Jalramet, Reaptan, Aspavor, Chela-fer and Myoprin?

I want to understand what are the contraindications and side effects caused in diabetic patients who are on chronic medication (Stillnox and Serdep in patients on Jalramet, Reaptan, Aspavor,...

25 June 2024 984 0 View

Hello, currently I am working to develop biopesticide product use Metarhizium. I would like to know how to formulate it so it can be effective?

Currently, I am working in oil palm plantation industry. This Metarhizium will be used to control the population of pest Oryctes rhinoceros. I need clue to formulate Metarhizium so it can be...

24 June 2024 9,926 3 View

I need to buy reducing agent Bruggolite FF6 M, how can I buy?

I have searched for CAS no. but cannot find.

24 June 2024 2,737 2 View

What is the potential role of phytochemicals in the prevention and management of asthma symptoms?

Asthma is a chronic respiratory condition marked by inflammation and narrowing of the airways. While traditional treatments involve medications like corticosteroids and bronchodilators, there's...

14 June 2024 8,063 2 View

For nuclei extraction from human cells, can I replace EGTA with EDTA in lysis buffer?

I understand that there are differences in ion preference as far as chelation is concerned when comparing EGTA vs EDTA. But is it ok to replace EGTA in my lysis buffer with EDTA since EDTA...

10 June 2024 1,188 1 View

How can I avoid interaction of plant based reducing agents to final nanocomposite for in situ preparation og Ag doped GO-Fe3O4 nanocomposite?

Can anyone comment on the following concerns I want to synthesize Ag doped GO-Fe3O4 nanocomposite in situ using plant extract as green method for Ag+ reduction. What will be the effect of plant...

23 May 2024 5,244 2 View

What concentrations should be selected for hemolytic assay for antimicrobials?

I am performing hemolysis assay for antimicrobials but there's difficulty in selecting the concentrations of the antimicrobials. I have read the papers and MHC 90 was determined. Should I consider...

21 May 2024 8,912 3 View

How to know if complexed Mn(II) has been (air-)oxidized to Mn(III)?

We have synthesized a polymer that attains a fairly good removal of manganese ions from synthetic waters contaminated with MnCl2. After many tests, we have deemed it a decent adsorbent. All in...

14 May 2024 1,811 2 View