Dear Researcher,
I am working on HIT_T 15 cells and I want to measure the intracellular calcium concentration using Fura_2am,
I found many protocols using probenecid ,
My question: is it ok if i use probenecid (water insoluble) but can dissolve in chloroform , NAOH ,etc..
and if anyone has a clear protocol kindly share with me will be appreciate
thank you