Dear Researcher,

I am working on HIT_T 15 cells and I want to measure the intracellular calcium concentration using Fura_2am,

I found many protocols using probenecid ,

My question: is it ok if i use probenecid (water insoluble) but can dissolve in chloroform , NAOH ,etc..

and if anyone has a clear protocol kindly share with me will be appreciate

thank you

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