I made a mistake, I seeded my cells in 6 well plates instead of 96 well plates.
my question is can I transfer my cells to 96 well by using trypsin
is that possible?
Is there any free software for caring out these calculations.
21 October 2023 4,961 0 View
this will be done to knoe fibre composition
19 September 2023 8,199 2 View
Hi everyone, I’m using hemoglobin in the lab and I need it to be in the reduced state (so it is able to bind with CO2). The hemoglobin I have is a lyophilized powder from Sigma Aldrich (H7379)....
13 September 2023 9,133 4 View
Hello, I need to use guanidium chloride to denature a protein. However, I’m reluctant because it is absolutely crucial for my experiment that no isocyanic acid is formed. I need to know the...
13 September 2023 4,587 1 View
Hi everybody, I have a questionnaire with 4 point Likert scale questions on participant experience. I want to score the results into low/moderate/high experience categories. How do I do this on...
15 June 2023 414 3 View
Please suggest any other method for estimating Heterogenous Panel Vector AutoRegressive thus allowing individual irf to be computed.
12 June 2023 9,061 3 View
Dear Researcher, I am working on HIT_T 15 cells and I want to measure the intracellular calcium concentration using Fura_2am, I found many protocols using probenecid , My question: is it ok if i...
17 April 2023 6,450 0 View
The molecular weight of EDTA is 292.24g/mol Thanks in advance!
01 February 2023 5,421 3 View
I'm working on peptides from frog skin secretions, and I need to measure the concentration of [Ca2+]! release from HIT-T15 cells. I'm confused about the proper protocol for [Ca2+]! assay by using...
12 May 2022 786 1 View
using fluorescence intensity? using ImageJ or any other software?
23 January 2022 3,266 3 View
I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...
02 August 2024 1,178 3 View
I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...
30 July 2024 2,301 7 View
Hello all, Recently as part of a research project at university, we have been studying the effects of light and temperature on eucalyptus seed germination. In our initial run of trials we...
24 July 2024 8,850 4 View
Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...
20 July 2024 4,143 2 View
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
11 July 2024 5,669 5 View
I have protein percentage and Seed yield per hectare data, how to calculate protein content kg per hectare.
11 July 2024 9,045 4 View
I am currently testing the effect of bacterial filtrates on cancer cells , after seeding the cells I tested the bacterial filtrates against them , and got images of all 96 wells using inverted...
10 July 2024 7,145 2 View
I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...
07 July 2024 407 0 View
I have attached pics of the SH-SY5Y cells within a seeded plate after reaching >80-90% confluence. Are these dark clumps of multiple growing cell layers? Or dead cells? Any clue? many thanks.
01 July 2024 8,361 3 View
Hi everybody, i need to pick your brains: i work with an adhesive fish cell line which forms a konfluent monolayer. I want to test an adsorbant substance, therefore i want to saturate the cell...
30 June 2024 9,300 0 View