How does a Bi-Static FMCW radar detect a Zero Doppler target?

27 September 2020 8,370 6 View

What is the best way to extract phenols from plants ?

What is the best way to extract phenols from plants ?What is the best way to extract phenols from plants without any loss

27 May 2020 9,005 17 View

Problem on reliability statistics - negative Cronbach's alpha?

In my study I want to see whether 3 items in a questionnaire relate to autonomy through a reliability analysis. The following three items are relevant I think: - Availability of task enrichment...

25 July 2018 3,223 2 View

What techniques can be used to locate fractures in underground metal water delivery pipes?

I recently met with an senior executive of a Water delivery company. #1 concern was the inability to locate leakage in underground mains pipes. Typically 40% of water is lost between source and...

24 May 2018 4,311 3 View

Would you like to participate in studies involving surgical outcomes, NSQIP, NIS, SEER, etc?

Seeking co-authors/collaborators to participate with me in database studies involving NSQIP, NIS, SEER, NAMC, etc. This is a good opportunity for post-docs and research fellows who can work...

04 November 2017 6,424 2 View

I am using simply P total RNA extraction kit (Bioflux, china) to extract total RNA from tissue sample do it require addition of mercaptoethanol

Does it require addition of lysis buffer or mercaptoethanol before homogenization or it's included in components of the kit reagents?!

03 August 2017 2,599 4 View

How many kinds of distance defined on graph in graph theory?

04 April 2017 4,384 13 View

How can I dissolve sodium palmitates in pure ethanol?

18 June 2016 784 3 View

Cell size units in point to raster?

I have metadata in my .dat file that tells me that my cell size is 0.200 degrees longitude. I want to import this file into GIS and convert my points to raster. My file is very simple: it has x, y...

29 September 2015 7,692 3 View

How can we assess the amount of sand available in a river and a lake?

What are the measures to be taken for controlled sand mining. Excess sand mining has become a big problem due to rapid urbanisation and development. Uncontrolled sand mining has now resulted in...

30 January 2014 5,444 1 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Is it possible to use maize mature seed to do transformation for maize transgenic study?

Almost all research reports which i ever seen work on Early mature embryo for Agrobacterium mediated maize transformation. In other grass families like rice, we can use mature seed and what do...

17 February 2021 1,434 13 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Seeds harvest from white clover by selfing?

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...

04 February 2021 9,152 4 View

What is the best method of preparing cell supernatant samples for a Luminex assay to detect cytokines ?

I am planning to run a Luminex assay using cell supernatant to assess cytokine secretion by tumor cells. I came across different methods in literature; some suggest seeding in complete media and...

03 February 2021 4,867 3 View

How do I measure Arabidopsis germination rate?

Hi there, I'm looking for a way to measure the Arabidopsis germination rate, like what media I should use to plant the seeds and what indicator I should see for germination. I'm planning to...

02 February 2021 4,785 3 View