Using multiple linear regression to solve a multiple-way ANOVA problem?

Specifically, I want to know if I can use multiple linear regression to predict a response value 'y' from it's associated "treatment" or "group" averages in several variables. Let's say:...

04 November 2020 632 19 View

Somebody know a fast DNA extraction method for plant for to do in the field?

I am looking found reference about people that has been working the proposed method in this publication: "Nucleic acid purification from plants, animals and microbes in under 30 seconds" from Zou...

10 March 2020 860 2 View

Do viruses mutate more when transmitted to another host than when staying within the first host?

I would like to know if there is a published work studying the within-host mutation rate of a virus versus the between-hosts mutation rate, when a direct transmission event occurred between those...

13 November 2019 5,488 5 View

No title

I have generated a stably transfected SK-N-SH cell line that expresses Amyolid beta 42 fused to the pre-proncephalin signal peptide under de CMV promoter. I checked expression though qPCR and...

23 July 2019 2,685 2 View

Is there a way in matrix algebra to define the operations you want to perform on matrices???

Let's say I want to perform the conventional matrix multiplication, so I define it as "multiply then sum the products" (i.e. "x,+") For example, for matrices A and B: if A = |a b| .........|c...

21 February 2018 6,474 3 View

How to perform an ELISA sandwich with Chylomicrons?

18 September 2017 8,336 8 View

How to perform an ELISA sandwich with Chylomicrons?

08 September 2017 4,123 8 View

How to calculate mass of dry Tris base + Tris HCl to dissolve to give a buffer with desired concentration?

I am trying to make a 1M Tris Cl pH8 buffer with dry Tris base and Tris HCl. I know that it is possible to make Tris buffers without the use of HCl by combining powdered Tris acid with Tris base....

02 April 2017 695 4 View

Which are the mechanism that could increase the number of d(GT) repetition in DNA?

How GT dinucleotides repetitions are increased in an microsatellite? Is the replication machinery involved in this phenomenon?

16 January 2017 4,612 2 View

How to void signal from digestive enzymes producing chemiluminiscence in immunoblots?

Hello! I did some in vitro digestions according to the INFOGEST (Minekus) protocol. When I analysed by immunobloting the different aliquots of the digestion I observed "background" signals that...

11 December 2016 6,422 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View