Patricia Román-Carrasco

4 Questions 5 Answers 0 Followers

Questions related from Patricia Román-Carrasco

How to perform an ELISA sandwich with Chylomicrons?

Hi! I am trying to develope a sandwich ELISA to detect a molecule in chylomicrons produced by Caco-2 cells. I normally coat the ELISA plate with anti-ApoB antibody, block with serum albumin, then...

09 September 2017 4,228 8 View

Bio-Rad Criterion tris-tricine gel electhophoresis. Which buffer to use?

I have to use a Bio-Rad Criterion tris-tricine gel... but I am not sure if I should use the cathode/anode buffers needed for a tricine-gel electrophoresis or instead, the single buffer described...

04 April 2017 9,920 3 View

How to void signal from digestive enzymes producing chemiluminiscence in immunoblots?

Hello! I did some in vitro digestions according to the INFOGEST (Minekus) protocol. When I analysed by immunobloting the different aliquots of the digestion I observed "background" signals that...

12 December 2016 6,518 2 View

Could someone help me to figure out what am I doing wrong in my blots?

Many of my immunoblots look like the ones in the attached photo with that strange form in the middle I tried different things during the handling but they still appear. Any suggestion? Thanks in...

02 February 2015 1,521 3 View