Best assay for cytotoxcicity dertemination in 3D culture?

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Malcolm Nobre

Hello Alja Štern

Please refer to the link below for the cytotoxicity determination in 3D culture for high throughput screening.

https://www.upmbiomedicals.com/resource-center/application-notes/high-throughput-cytotoxicity-testing-with-hepg2-cells-grown-in-3d-culture/

Hope it will help!

Best.

Arash Abdolmaleki

Dear Alja

Best and also cheap test for cytotoxcicity dertemination is MTT test. In this method it is better that remove scaffolding before reading the plates by Elisa reader.

Asadollah Asadi

Dear Researcher

Viability of 3D culture seeded cells is achievable via MTT assay on scaffold/ after removing scaffold. Application of scaffold free procedure may limit restrictions related to Microplate reader noise caused by scattering or turbidity problem.

Lucas Santos

Hello, Alja!

For 3D culture, the MTT assay is widely used. For 2D culture, flow cytometry is used a lot to verify viability. However, I don't know if it is recommended for 3D culture.