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Questions related from Alja Štern
We are trying to measure intracellular H2O2 in spheroids post exposure to nanozymes, that should generate ROS, using the ROS-Glo kit from Promega. However, we are getting strange results. The...
10 June 2024 2,804 0 View
I am searching for the best approach to statistically evaluate differences in cell cycle distribution between different cell populations. We are analysing the cell cycle distribution, by staining...
04 January 2023 7,716 5 View
What would be the best assay for cytotoxcicity dertemination in 3D culture? It should also be quite cheap for screening purposes.
22 February 2022 5,995 4 View
It was commonly accepted in during my student years in the lab I was working, that when you add the MTT reagent (which is toxic to the cells), the cells die. So my question is if they do, how long...
18 February 2022 4,559 2 View
Has anyone a suggestion for a good Ca2+ detection colorimetric assay kit (96well) for measuring Ca2+ in very complex growth media containing lots of protein, minerals and oils... We used to...
21 November 2018 3,947 1 View
We are measuring filamentous bacterial biomass in bioprocesses using a capacitance probe (biomass monitor from Aber). In the log phase the biomass signal is growing, as is the viscosity of the...
27 October 2018 2,225 1 View
Is it possible to get rid or at least reduce the concentration of sulphate in water in a simple, low cost way, that would be suitable for large amounts of water, without too much disturbing the...
19 August 2018 7,862 2 View
I would like to get a quick estimation of total Ca content in soy concentrates. I was thinking that by acidifying and heating I can solubilize the majority of the Ca and measure it with an ion...
30 March 2016 3,421 5 View
I got samples of different enzymes in liquid form and the specification only gives the activity in U/g but I can’t figure out whether that means per g substrate proteine, per g enzyme or per g of...
18 November 2015 6,674 6 View
Witch wavelength is best for measuring protein in the Biuret test? I came across different variants ranging from 540-565nm, with the reagent consisting of the same chemicals. I tried measuring at...
10 November 2015 5,316 3 View
