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We are trying to measure intracellular H2O2 in spheroids post exposure to nanozymes, that should generate ROS, using the ROS-Glo kit from Promega. However, we are getting strange results. The...
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I am searching for the best approach to statistically evaluate differences in cell cycle distribution between different cell populations. We are analysing the cell cycle distribution, by staining...
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What would be the best assay for cytotoxcicity dertemination in 3D culture? It should also be quite cheap for screening purposes.
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It was commonly accepted in during my student years in the lab I was working, that when you add the MTT reagent (which is toxic to the cells), the cells die. So my question is if they do, how long...
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Has anyone a suggestion for a good Ca2+ detection colorimetric assay kit (96well) for measuring Ca2+ in very complex growth media containing lots of protein, minerals and oils... We used to...
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I would like to get a quick estimation of total Ca content in soy concentrates. I was thinking that by acidifying and heating I can solubilize the majority of the Ca and measure it with an ion...
30 March 2016 3,375 5 View
I got samples of different enzymes in liquid form and the specification only gives the activity in U/g but I can’t figure out whether that means per g substrate proteine, per g enzyme or per g of...
18 November 2015 6,618 6 View
Witch wavelength is best for measuring protein in the Biuret test? I came across different variants ranging from 540-565nm, with the reagent consisting of the same chemicals. I tried measuring at...
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