We are trying to measure intracellular H2O2 in spheroids post exposure to nanozymes, that should generate ROS, using the ROS-Glo kit from Promega. However, we are getting strange results. The Blank, negative control and solvent control are sometimes higher than the samples with the nanozymes. The negative control contains only complete cell media, the solvent control is 2% MillQ in complete media, and the samples are also in complete cell medium. the blank si complete medium without cells. We tried measure different media compositions, from minimal to complete, without cells, but the measurements didn't make sense. Has anyone experienced similar?
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