First time secondary research, some points I should keep in mind?

I am conducting secondary research for my dissertation for undergraduation (Psychology) , what should I make sure my proposal consists of? Are there any tips I need to keep in mind?

22 February 2021 6,035 1 View

How to interface ANSYS Maxwell and Matlab?

Dear All, I am working on electric motor design in Ansys Maxwell. I have written optimization code in MATLAB. I want to run this code in ANSYS MAXWELL and generate FEA results. Does anyone know...

10 February 2021 1,279 1 View

I have faced a problem to amplify VP2 gene of porcine parvovirus 1,2,3 and 5? Please suggest me how can i solve this problem?

Till now i haven't got any PCR amplified vp2 gene bands on specific annealing temperature and in step up PCR.

08 February 2021 819 4 View

Can anyone provide me with the scoring method and the questionnaire of Work life balance scale by Hayman (2005)?

Greeting! For my MA dissertation, I am thinking of taking Work life balance scale by Hayman. However, I can't find the scoring procedure; also confused about the actual rating taken. If anyone...

23 January 2021 4,074 7 View

Quantitative Research Involves 3 Different Likert Scales, how do I go about data analysis?

This is our first research paper and it is titled "Effect of Peer Connection on Attitude Towards School and Academic Motivation in Higher School Students During Pandemic". We aim to investigate...

30 December 2020 5,450 4 View

My research involves 3 diff scales about peer connect, attitude towards school and academic motivation, how should I go about finding the correlation?

This is the first time I'm conducting research and I'm unsure about the result and scoring process, kindly help

27 December 2020 1,260 3 View

Hi everyone, I am digesting my plasmid with SgrAI enzyme but it showed staractivity everytime?

My plasmid (pJET1.2) contains an insert of around 2969bp. The total size of plasmid 5942 bp. I am cutting it with AhdI, SgrAI and BssHII. Desired bands are of 2969, 1632 and 1432bp. I want to...

15 November 2020 4,761 4 View

Perceived Academic Stress?

Hello, Does anyone have any idea of how this is scored? What would be the average scores? Would would be seen as very low/high? BEDEWY D & GABRIEL A. Examining perceptions of academic...

09 November 2020 2,040 1 View

Does Shannon index represent largest diversity index when the individual belongs to different species and vice versa?

The shannon wiener index and Simpson indices include the measurement of community heterogeneity. Shannon wiener index bapplied information theory. The main measuring object of information theory...

22 October 2020 1,053 7 View

How to write an effective and attractive title for research paper?

I want to get the suggestions, observations and experiences of different researchers around the world for an effective and attractive research paper title.

15 October 2020 6,411 28 View

Interpreting and troubleshooting anomalous HPLC results?

I am running a simple isocratic Shimadzu HPLC system coupled to a fluorescence detector to detect and quantify biogenic amines from in vivo samples. But my results are not as expected and I'm...

10 November 2020 7,027 5 View

What detector(ELSD, UV, RI) to use for poly unsaturated fatty acid column chromatography? Using a Teledyne ISCO with silica gel column. Suggestions?

Column chromatography with Silica Gel columns with inbuilt UV detector. Compound structure is mainly poly unsaturated fatty acids and analogs without UV handles.

22 March 2020 3,445 4 View

Does anyone have experience with fMLP as a neutrophil chemoattractant in a transmigration assay?

I am planning to perform a transmigration assay (transwell plate) in which are involved neutrophils , therefore I will use fMLP as a chemoattractant. I did find some papers that describe "The...

19 February 2020 5,855 3 View

How to do kinetic assay with nitrocefin? Is there any particular buffer other than phosphate buffer?

03 February 2020 1,487 3 View

How to do kinetic assay with nitrocefin? Is there any particular buffer other than phosphate buffer?

01 February 2020 9,280 3 View

What is the mechanism behind Fast and Slow EPSCs?

I know for fast EPSC non-NMDA channels are involved and similarly, slow EPSC is mediated by NMDA channels. But what's the actual mechanism underlying these processes?

01 February 2020 7,933 7 View

What is the best way to cleave GAGs (HS/CS) from protein samples for western blotting?

I have Heparanase III and Chondroitinase ABC which I would like to use to cleave the heparan sulfate and chondroitin sulfate chains off their core proteins before I run the samples on a western...

27 January 2020 496 1 View

Is there any protocol to extract phenolics compound prior to protein extraction?

I am trying to extract N-glycosylated proteins starting from 10 g of frozen plant lmaterial but I am facing a real problem with phenolics and oxidations. The protein solution is very dark...

06 January 2020 3,534 14 View

What is the best way to cleave GAGs (HS/CS) from protein samples for western blotting?

I have Heparanase III and Chondroitinase ABC which I would like to use to cleave the heparan sulfate and chondroitin sulfate chains off their core proteins before I run the samples on a western...

31 December 2019 1,792 1 View

Is there any protocol to extract phenolics compound prior to protein extraction?

I am trying to extract N-glycosylated proteins starting from 10 g of frozen plant lmaterial but I am facing a real problem with phenolics and oxidations. The protein solution is very dark...

31 December 2019 3,849 14 View