Besides SDS-PAGE, is there a method to detect the presence/absence of protein in samples subjected to N-glycan method? - FAQS.TIPS

Harleen Kaur @Harleen-Kaur-57

29 July 2019 1 6K Report

Hi,

Besides SDS-PAGE, is there a method to detect the presence/absence of protein in samples subjected to N-glycan method?

Regards,

Harleen

Harleen Kaur

Hi Stjepan,

Yes, I'm referring to PNGase F treatment of N-glycans.

Regards,

Harleen

Badges
Science method

More Harleen Kaur's questions See All

How to draw a graphs of bioconcentration of pesticide and its relationship with log Kow, log Sw and molecular weight?

Help me to draw graph of Bioconcentration

10 June 2024 4,941 2 View

How to estimate total sugars using lane and eynon method?

I want to estimate total sugars in carrot and products developed from it. I have been using lane and eynon method. I could get the values for reducing sugars but not for total sugars. What is the...

28 May 2024 9,382 4 View

Dissertation topic for MSC Computer Engineering Students?

My name is kiranbir kaur and m student of MSC Computer Engineering in Solent University Southhampton (United Kingdom).Please suggest me the topic for dissertation.Include the Problem and Solution.

24 May 2024 4,355 2 View

Dissertation Topic for MSC computer Engineering?

Hi, My name is kiranbir kaur and m student of MSC Computer Engineering in Solent University Southhampton (United Kingdom).Please suggest me the topic for dissertation.Include the Problem and Solution.

24 May 2024 1,180 3 View

Why total dissolved solids (TDS) are not reduced after treating waste water by vermifiltration process?

Dear researchers, I am treating the waste water by vermifiltration process using different filter bed media. After the treatment, BOD, COD, TSS were found to be reduced but reduction in total...

18 May 2024 1,897 3 View

Why do atoms make bond?

what is the main reason of formation of bonding in between two atoms

16 May 2024 3,607 0 View

What formula we use for calculation of anthocyanin from optical density values?

Using 10 ml of 1% methanolic hcl, 1 g fresh sample and OD at 530 and 657nm

07 May 2024 4,440 1 View

Can anyone advice me on LncRNA primer designing for cloning to study its overexpressed pattern in the cell?

I am planning to study the overexpressed pattern of lncRNA. Could you explain whether the primer designing procedure for cloning a long non-coding RNA (lncRNA) gene follows the same approach as...

14 April 2024 3,966 3 View

Hello everyone i have some problem when i am installing cooja 3.0 in ubuntu 18.04? give me some solution?

when i am installing cooja 3.0 in ubuntu 18.04 i am facing some problem give me some solution

01 April 2024 4,305 0 View

Is there any method by which we can test the anticancer property of the compound, without using any cells or cell line?

I have searched for the methods where I do not require any cell or cell line, but I could not find it. I want to check the anticancer property of the given compound (protein) without using cells....

21 March 2024 10,021 3 View

Similar questions and discussions

Is it possible that PNGase F does not cleavage all glycans?

The NS1 protein of the tick-borne encephalitis virus potentially has three glycosylation sites. When processing the PNGase F protein on SDS-PAGE, I observe three bands - a fully glycosylated 52...

01 August 2024 3,519 4 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

What types of graphs are commonly used when wanting to visualize data on lectin microarrays and glycans?

I would like to know what types of graphs are commonly used when wanting to visualize data on lectin microarrays and glycans. The goal is to compare samples from patients with lung cancer and...

21 February 2024 7,127 1 View

How to cleave the glycans on SARS-CoV-2 spike proteins?

Hi all, For the imaging of fine structure on the surface of spike proteins, I want to try to cleave glycans on the protein surface. Is there any enzyme/method that can be used to do it without...

27 October 2023 3,798 0 View

Why we will caculate equilibrium potential of chlorine, when we record mEPSC?

In the process of voltage clamp recording, cesium internal solution was commonly used in our laboratory. In general, we used high-chloride cesium internal solution to record mEPSC and mIPSC, but...

16 August 2023 4,451 2 View

How to generate the force field for the modified monosaccharide residue in AMBER?

I am using GLYCAM in AMBER to conduct simulations of glycans. But the system I am working on contains modified monosaccharide residues GlcNAc6S. Basically, there is a sulfate group attached to C6...

20 May 2023 5,347 1 View

Which fatty acids can be converted to eicosanoids?

Can all unsaturated fatty acid be converted to eicosanoids?

13 February 2023 7,992 2 View

Is it possible to use lectin as an antibody to block some specific glycans?

Hello everyone, I would like to block some specific glycan (Gal & GalNAc) on the surface of the cells to investigate their physiological roles, in particular for cell adhesion and cell-cell...

12 February 2023 5,540 0 View

Where are fatty acids converted to eicosanoids?

Eicosanoids are generated from fatty acids but I was wondering where this conversion takes place? In the cells or outside of the cells?

01 February 2023 6,039 1 View

Are there other (free) database for MS-glycan analysis, similar to reaxys?

Hey everybody, I would like to use a database for massspectometry based oligosaccharide analysis derived from human tissue. So far I only found Expasy GlycoMod and Glyconnect. Both of them work...

26 January 2023 6,710 0 View