I am planning to study the overexpressed pattern of lncRNA. Could you explain whether the primer designing procedure for cloning a long non-coding RNA (lncRNA) gene follows the same approach as for protein-coding genes, or are different strategies employed specifically to study the overexpression patterns of lncRNAs?
Hi Pooja
re is a good paper from a classic mRNA to a specific lncRNA nothing change in the primers designing for cloning. as other cloning procedures, you just need to be sure to clone all regulatory sequence to ensure its expression in the infected cells.e
from a classic mRNA to a specific lncRNA nothing change in the primers designing for cloning. as other cloning procedures, you just need to be sure to clone all regulatory sequence to ensure its expression in the infected cells. There is a good paper in MMB which could help you:
Article Methods for the Study of Long Noncoding RNA in Cancer Cell Signaling
all the best
fred
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