How to cleave the glycans on SARS-CoV-2 spike proteins?

28 October 2023 0 4K Report

Hi all,

For the imaging of fine structure on the surface of spike proteins, I want to try to cleave glycans on the protein surface. Is there any enzyme/method that can be used to do it without destroying the trimer structure? Thanks!

Badges
Science topic

Similar topics
Social Science
Media
Journalism

More Pangmiaomiao Zhang's questions See All

Do experts have journals in the field of artificial intelligence and big data that are not indexed by SCI or EI?

05 August 2024 8,836 2 View

Are there any good simple systems or platforms to recommend?

In order to show people the beauty of control and enhance enthusiasm for learning control theories, are there any good simple systems or platforms to recommend?

05 August 2024 10,034 1 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

The complex interactions between oral microbiota and oral mucosal immunoregulation: implications for oral health and disease development?

This research question explores the complex interactions between oral microbes and oral mucosal immune regulation. The diversity and function of oral microbes have important impacts on oral health...

09 July 2024 7,883 0 View

How to solve the problem that the film obtained by magnetron sputtering on the ternary alloy target A2BC is ABC (not A2BC)?

Using magnetron sputtering with an alloy target composed of A:B:C in a 2:1:1 ratio, the resulting thin film composition is A:B:C=1:1:1. How can you achieve a A:B:C=2:1:1 composition? What are some...

24 June 2024 1,605 0 View

A small question about the principle of lasers…?

Given the energy level diagram of a ruby laser shown in the right figure, where \( R_{02} \) is the pumping rate, and \( \tau_2 \) and \( \tau_1 \) are the lifetimes of levels \( n_2 \) and \( n_1...

23 June 2024 4,855 0 View

Can the matter wave theory which suggests that when matter waves resonate be used to solve HTS ?

Can the matter wave theory, which suggests that when matter waves resonate, the energy input from the outside is minimal, be used to solve all superconductivity problems? The matter wave theory,...

17 June 2024 7,957 0 View

There is any relationship between critical temperature of superconductor and the atomic weight & radius?

till now, there are many superconductors. Is there any relationship between critical temperature of superconductor and the atomic weight & radius? Now the isotope effect is one relationship...

12 June 2024 1,908 3 View

How to promote an academic journal and get high-quality manuscripts?

International Journal of Complexity in Applied Science and Technology(https://www.inderscience.com/jhome.php?jcode=ijcast) is a new journal. Can anyone tell me how to promote the new journal and...

09 June 2024 1,922 1 View

DNA FISH with multiple signal spot ?

I am doing DNA FISH on mice stem cells to detect an endogenous gene location. The expected result should have two signal spots, representing the two copies of the allele. However, what I see is...

06 June 2024 941 1 View

Is it possible that PNGase F does not cleavage all glycans?

The NS1 protein of the tick-borne encephalitis virus potentially has three glycosylation sites. When processing the PNGase F protein on SDS-PAGE, I observe three bands - a fully glycosylated 52...

01 August 2024 3,519 4 View

What types of graphs are commonly used when wanting to visualize data on lectin microarrays and glycans?

I would like to know what types of graphs are commonly used when wanting to visualize data on lectin microarrays and glycans. The goal is to compare samples from patients with lung cancer and...

21 February 2024 7,127 1 View

How to generate the force field for the modified monosaccharide residue in AMBER?

I am using GLYCAM in AMBER to conduct simulations of glycans. But the system I am working on contains modified monosaccharide residues GlcNAc6S. Basically, there is a sulfate group attached to C6...

20 May 2023 5,347 1 View

Is it possible to use lectin as an antibody to block some specific glycans?

Hello everyone, I would like to block some specific glycan (Gal & GalNAc) on the surface of the cells to investigate their physiological roles, in particular for cell adhesion and cell-cell...

12 February 2023 5,540 0 View

Are there other (free) database for MS-glycan analysis, similar to reaxys?

Hey everybody, I would like to use a database for massspectometry based oligosaccharide analysis derived from human tissue. So far I only found Expasy GlycoMod and Glyconnect. Both of them work...

26 January 2023 6,710 0 View

How to create an itp GROMACS file from the tpr?

Dear ResearcherGate community, I created a Coarse Grained system composed of a glyco-protein using the MARTINI force field and GROMACS files. Both sugar and protein parameter files were created...

04 August 2022 9,974 0 View

Does azido labelling have any advantages over antibodies when looking for presence/absence of certain glycan-linked molecules?

Hi everyone, Suppose I want to look for glycan-bound (specifically O-acetylglucosamine-bound) molecules, such as certain proteins or lipids, in a cell sample. In this scenario, I am not interested...

16 May 2022 5,997 0 View

How to deglycosylate intact live immune cells?

Hi everyone and expert glycobiologists! I'm trying to optimize an assay that uses PNGaseF (N-glycosidase; New England Biolabs P0705S) to deglycosylate my cells (Jurkat T-cells). However, I cannot...

04 May 2022 1,493 0 View

What are the possibility that methanol also precipitates the glycans along with protein ?

In the N-glycomics experiment I am unable to see any glycans nor any protein. I am doubting whether it could be the separation step of protein and N-glycans might be the problem. Actual...

21 March 2022 3,522 3 View

What standard to use to analyse mouse IgG N-glycans by capillary electrophoresis?

I would like to analyze the N-glycosylation profile of murine IgG isolated from the serum by capillary electrophoresis. To assign a specific glycan for each of my peaks after the run, I have to...

14 December 2021 2,915 2 View