Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

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23 February 2021 4,014 3 View

Build an orthogonal box in ASE?

Hello, I was wondering the best way to build an orthogonal box filled with a defined crystal structure in ASE. I know it could be easily done in LAMMPS, but I guess it is also doable in ASE. The...

21 February 2021 5,899 4 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

How to find a particular aptamer complementary sequence?

I can find several papers that give me a guide for choosing a suitable aptamer sequence 1. But how to find a property aptamer sequence 2(partially complementary to sequence 1)?

09 February 2021 3,511 1 View

Do anyone have a full MATLAB code for structural topology optimization using Genetic Algorithm? Such as a cantilever beam?

Do anyone have a full MATLAB code for structural topology optimization using Genetic Algorithm? Such as a cantilever beam? Could you provide some MATLAB code to me , a beginner for GA. Thank...

03 February 2021 3,789 5 View

How to do DNA normalization based on qPCR values?

Hi Everyone: I have a question regarding to qPCR. I am trying to do RT-PCR, and I want to have equal amount of input DNA for my RT-PCR, so I normalize my DNA input based on qPCR values of a...

31 January 2021 6,977 4 View

MS/MS protein analysis?

Hi Everyone: I want to quantify the relative amount of a protein in 2 samples. I will first digest the sample with trypsin, and subject the resulting peptides to LC-MS/MS analysis. I will then...

25 January 2021 8,333 3 View

What is the best way to cleave GAGs (HS/CS) from protein samples for western blotting?

I have Heparanase III and Chondroitinase ABC which I would like to use to cleave the heparan sulfate and chondroitin sulfate chains off their core proteins before I run the samples on a western...

27 January 2020 496 1 View

Is there any protocol to extract phenolics compound prior to protein extraction?

I am trying to extract N-glycosylated proteins starting from 10 g of frozen plant lmaterial but I am facing a real problem with phenolics and oxidations. The protein solution is very dark...

06 January 2020 3,534 14 View

What is the best way to cleave GAGs (HS/CS) from protein samples for western blotting?

I have Heparanase III and Chondroitinase ABC which I would like to use to cleave the heparan sulfate and chondroitin sulfate chains off their core proteins before I run the samples on a western...

31 December 2019 1,792 1 View

Is there any protocol to extract phenolics compound prior to protein extraction?

I am trying to extract N-glycosylated proteins starting from 10 g of frozen plant lmaterial but I am facing a real problem with phenolics and oxidations. The protein solution is very dark...

31 December 2019 3,849 14 View

Does anyone have a protocol to purify glycosylated fibronectin from serum samples?

I am trying to purify glycosylated fibronectin from serum samples. Unfortunately, there is no specific antibody against glycosylated fibronectin. So, I want to use lectin-based affinity...

11 September 2019 1,426 3 View

Are there known factors/parameters affecting the sialylation/desialylation of recombinant glycoproteins produced in cell cultures?

Can a human recombinant glycoprotein produced in mammalian cell culture lose part of its sialic acid residues due to changing conditions in the culture or the purification of the protein? Any...

17 May 2019 9,954 3 View

Are there known factors/parameters affecting the sialylation/desialylation of recombinant glycoproteins produced in cell cultures?

Can a human recombinant glycoprotein produced in mammalian cell culture lose part of its sialic acid residues due to changing conditions in the culture or the purification of the protein? Any...

04 May 2019 5,218 3 View

Removal of glycerol from a glycan sample in water?

I have a sample of glycans which were removed via enzyme (this enzyme is glycerol-free), however my NMR spectrum showed a ton of glycerol. I want to recover the glycans, but remove the glycerol....

11 December 2018 6,890 3 View

Sodium cyanoborohydride in reductive amination of free glycans - how to handle reaction products?

I want to perform reductive amination of glycans using sodium cyanoborohydride as the reducing agent. Is there any advice on how to handle the reaction products, e.g. neutralise left over reducing...

31 December 2017 9,865 2 View

Why are mucins slimy?

Mucins are rich in o-glycans. What exactly make them slimy? (The chemical nature)

01 February 2017 3,814 0 View